antibiotics, or improper use. By determining the resistance and cross-resistance, one may determine the the relative ability for bacteria to gain resistance to antibiotics. We hypothesis that if we expose Enterobacter cloacae to Tetracycline over a week, then the bacteria will evolve a resistance and the zone of inhibition will decrease. Also, the bacteria will gain a resistance to other antibiotics and biocides, such as Triclosan.…
the tube but stayed rather stationary and near the bottom, showed that it appeared to be anaerobic, but this conclusion is inaccurate with my hypothesis, since Enterobacter aerogenes is considered to be “facultative” based on page 85 of the lab manual appendix E (MB 352 Laboratory manual pg 37, 41, 85). Additional testing was done in lab 5, from which we utilized tests with “SIM agar deep tubes, nitrate reductase, protein hydrolysis, catalase, and cytochrome oxidase” (MB 352 Laboratory manual…
Enterobacter spp. Enterobacter spp are part of the enterobacteriaceae family. They are facultative anaerobic gram negative bacilli. Their means of motility is by means of petrichous flagella. They produce acid upon glucose fermentation and are methyl red negative, voges-proskauer positive. Optimal growth temperature is 30*C .80% are encapsulated. E. spp have been associated with nosocomial outbreak and are opportunistic pathogens. They can cause numerous infections such as cerebral abscess,…
microorganism to be Enterobacter Aerogenes. The plan to identify the microorganism was to grow a pure and uncontaminated…
1) Alcaligenes faecalis is a Gram-negative coccobacilli that is motile, oxidase positive, catalase positive, non-spore-forming, non-fermenting, slow growing, and citrate positive obligate aerobe (Mahon et al., 2014) (Sachdeva et al., 1963). Its colony morphology is flat, thin, and rough with irregular edging (Mahon et al., 2014) and the flagella varies between the usual shape versus the curly structure, however, both shapes do not exist on the same individual bacteria (Leifson, 1960). The…
All of the isolates shown in Table 1 were resistance to S. aureus and were considered antibiotic producers. However, only isolate 12 and 16 showed resistant to the unknown Gram-negative organism. All of the antibiotic producers were then tested against Enterococcus faecalis, Pseudomonas putida, Acinetobacter baylyi, Escherichia coli, and Enterobacter aerogenes by the streak-plate method, which can be seen in Table 2. As demonstrated in Table 2, none of the antibiotic producers were found to be…
Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of…
et al., 2004). A. faecalis can not ferment lactose, so the colonies would be off-white (not red/pink) on MacConkey’s agar (Kavitha, n.d.). A. faecalis will have a positive results on Simmons citrate agar, indicated by the royal blue color (Kavitha, n.d.). This microorganism is negative for glucose and lactose fermentation (LA Mission College, 2009). A. faecalis would not grow on glucose minimal media because it cannot use glucose, also it would require high concentrations of salts (Kersters,…
Introduction Identifying microbes are important for working in the medical fields and also for research. Physical and cellular processes are ways in which microbes can be identified. A series of test were done to identify an unknown microbe labeled 5. Based on observation and the results conclusion was made to identify the bacteria as Enterobacter aerogenes. In microbiology, researchers are frequently trying to culture and classify microorganisms in a laboratory setting for study. Because of…
E. cloacae is a gram-negative, rod-shaped (bacilli) bacterium. It possesses flagella for motility and is a facultative anaerobic microorganism that is able to obtain energy from both aerobic and anaerobic environments (Britannica). E. cloacae is a nosocomial pathogen, more commonly contracted in the ICU. Enterobacter are ubiquitous in nature. Animals contain Enterobacter in their intestinal tracts allowing for further transmission of the bacteria in soil, water and sewage (Britannica). In…