ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) ELISA is a technique to detect and quantify substances like proteins, antibodies, and hormones. The target antigen is immobilized to the bottom of the well and then an antigen-specific detection antibody is added. The detection antibody makes it possible to measure results. The three most common formats of ELISA would be direct assay, indirect assay, and sandwich assay. In direct assay, the antigen immobilizes to the bottom of the well where a labeled antibody attaches to it. In indirect assay, the the antigen immobilizes to the bottom of the well and then a primary antibody binds to the antigen. After that, the labeled secondary antibody binds to the primary antibody. In sandwich assay, a pre-coated…

B, P. (2015) Evaluation of sensitivity and specificity ELISA AGAINST Widal test for typhoid diagnosis in endemic population of Kathmandu. BMC Infectious Diseases. 15:523. DOI 10.1186/s12879-015- 1248-6. This article compares and contrasts the sensitivity and specification of Enzym-linked Immunosorbent Assay (ELISA) and the Widal test for diagnostic purposes. To compare the two assays the researchers used blood culture collected from febrile patients for the last three years. The paper clime…

In this research paper, the functional relationship between protein named Albert and Alice will be prove using two advance techniques. Most of the molecular processes within the cell are carried out by protein-protein interaction (PPI), diseases such as cancer, Alzheimer’s disease can caused by abnormal PPI. As prior knowledge has advocate that this two proteins may have similar cellular pathway, therefore understanding if they have PPI becomes vital. There will be two methods described in this…

F. Gao et al., (2013) with minor modifications in dialysis and concentration of antibodies, were obtained using Amicon® Ultra - 4 10K centrifugal filter devices, checked for functionality and cross reactivity with heterogeneous snake venoms as mentioned in sandwich ELISA protocol and stored at 4oC. 3.11 Synthesis of colloidal gold Gold nanoparticles about 25 nm were prepared using modified citrate reduction method described by Grabar et al. (1995). Briefly, 200 ml of 0.01 % HAuCl4 solution…

Skill #28 Kirstin Krueger #21839116 ELISA is an abbreviation for "enzyme-linked immunosorbent assay." An ELISA test uses different parts of the immune system, different antibodies, and color changes to detect immune responses in the body. CPLi, or Canine Pancreatic Lipase Immunoreactivity, is a specific lipase that is only found in canine pancreatic tissues. The CPLi ELISA snap test is made by Idexx and is used to measure the concentrations of pancreatic lipase in the serum of the patient.…

determine the cell number. Finally, to quantify the cell number by stander curves, the optical density were plotting with the known cell numbers using linear stander curves. The cell numbers was adjusted by interpolating from a stander curve using prism software (R2=0.98). 4.2.3 Hormone analysis 4.2.3.1 Progesterone ELISA Analysis of progesterone in GCs supernatant is done by enzyme linked immunosorbent assay (ELISA). The assay was performed as described in section 3.2.2.2. P4 concentrations…

The SH-SY5Y neuroblastoma cell line was procured from National Centre for Cell Sciences, India and maintained in (DMEM) (Sigma Aldrich, USA) and Ham 's F12 nutrient mixture (F12) (Sigma Aldrich, USA) in 1:1 ratio. The medium was supplemented with 2 mmol L-glutamine (HiMedia, India), 10% (v/v) heat inactivated fetal bovine serum (FBS) (Hyclone Laboratories, Logan, UT) and 1% antibiotics (penicillin/streptomycin). Cells were cultured at 37 ºC in a humidified CO2 ¬incubator containing 5% CO2 and…

Griess reagent (Sigma-Aldrich Chemical Co.). For this assay, the supernatant was collected and mixed with the same volume of Griess reagent for 10 min at room temperature in the dark. The absorbance was measured at 540 nm using a microplate reader, and NO concentrations were calculated by referencing a standard curve generated from the known concentrations of sodium nitrite [Lee et al., 2015]. Measurement of PGE2, TNF-α, and IL-1β production in RAW 264.7 macrophages To measure the production…

1) ELISA - Enzyme-Linked Immunosorbent Assay i. What is the purpose of an ELISA? - The purpose of ELISA is to immobilize certain antigens and incubated them with antiserum so that the concentration of the antibody in the antiserum is quantified using an enzyme-linked anti-immunoglobulin antibody also known as an ELISA. ii. Explain in your own words the following types of ELISAs: direct, indirect, capture or “sandwich”? - Direct ELISA: The direct ELISA is a more simple method, in…

The mysterious passing of Elisa Lam, a twenty-one year old Canadian student, was one on the most famous unsolved deaths in history. The Cecil Hotel in Downtown Los Angeles, where Elisa was staying, received many complaints of yellow colored and “off tasting” water. They finally decided to inspect the water tank on February 19, 2013. During this inspection they were shocked to find the naked body of Elisa floating in the yellowing waters. It all began when Elisa checked into The Cecil Hotel…