DNA replication

DNA sequencing (Sanger to NGS) Introduction: The DNA sequencing methods have quickly evaluated for the forty last years and their finding was joined by breakthroughs in the field of Molecular biology (1). The first efficient method to sequencing DNA was found by F.Sanger in 1977 (2). But the huge desire to sequencing genomes required faster methods and many improvements were made. Fluorescence, capillary electrophoresis and microarrays led to a new way for sequencing genes : the Next Generation…

information as ‘’DNA makes the RNA and RNA makes the protein.’’All biological cells with few exceptions in some follow this rule. This flow takes place through three fundamental processes: replication, transcription and translation. Central Dogma theory was modified when it was found that in some cases RNA carry the genetic information for DNA. The dogma is basically the transfer of sequenced information between information carrying biopolymers. There are three main classes of such biopolymers:…

when in low concentrations, could be mutagenic and carcinogenic but another cause of human exposure to this type of agents is cancer chemotherapy, in which they are choose due to their ability to induce extensive DNA damage and cell death (9). They also can induce a great quantity of DNA lesions because they can react with different…

Papillomaviruses are thought to have two methods of replication: • Stable replication of the episomal genome in basal cells • Runaway, or vegetative, replication in more differentiated cells to generate progeny virus. “Viral multiplication is confined to the nucleus. Consequently, infected cells exhibit a high degree of nuclear atopia. Koilocytosis (from Greek koilos “empty”) describes a combination of perinuclear clearing (halo) with a pyknotic or shrunken (raisinoid) nucleus and is a…

Biology Matching Terms DNA- is the replication of your parents and ancestors genes RNA- is the transcription of dna, ribonucleic acid; guides protein synthesis. Translation- process in which RNA is synthesized from the template DNA. Transcription- process in which RNA attaches to the ribosome and a protein is assembled. Replication- method of DNA replication in which parental strands separate, act as templates, and produce molecules of DNA with one parental DNA strand and one new DNA strand.…

In the spring of 1953 James Watson and Francis Crick discovered the true structure of deoxyribose nucleic acid (DNA) utilizing theoretical chemistry and borrowed X-ray crystallography images. They postulated that the structure of DNA contained two helical chains that run in opposite directions of one another. They also stated, in contrast to Linus Pauling’s structure, that due to the repulsive forces between the negatively charged phosphate groups the nitrogenous bases would be located on the…

A, B and Z DNA Structure These three types of DNA all possess the similar property of a double helical structure formed from a sugar-phosphate backbone and containing the 4 bases, adenine, thymine, guanine and cytosine, complementarily paired with hydrogen bonding. A and G are purines and have a double ring whereas T and C are pyrimidines and have only a single ring. Within the helix a pyrimidine is always paired with a purine such that G is always paired with C and T with A. The difference…

processes in DNA replication, their existence reduces the likelihood of a mutation occurring by a significant amount. In fact, proofreading reduces the likelihood of errors occurring to approximately one in every ten million nucleotides (Pierce, 2012-pp354). This essay will emphasise the importance of proofreading during, and mismatch repair post DNA replication and explore its significance in the mechanisms that drive Lynch syndrome. In eukaryotes 3 DNA polymerases are used for Elongation; DNA…

error-free post-replication repair by contributing to polyubiquitylation of proliferating cell nuclear antigen (PCNA). Up-regulation (12.52-fold) of Rad8 indicated that cold and freeze shock caused DNA damage in the cells, Rad8 was expressed to carry out the repair mechanism in order for the cells to survive. Other DNA damage repair proteins, such as cdc36, and NER were expressed when cells were exposed under cold stress, this observation indicated that rapid cold caused severe damage to the…

The RNA worlds together with the DNA world are known to have numerous differences as well as similarities with the cell nature as well as the varied virus character demands pointing to the viruses coming first. To a degree, the theories that designate this phenomenon have contended on the dissimilar nature of viruses, as a crucial factor that illustrates the viruses coming first (Woese, 1987). In the biosphere of microbes, whereas viruses are regarded as minuscule in size, the Pithovirus is…