Denaturation

PCR History The invaluable molecular technique known as PCR, or the polymerase chain reaction, was discovered by a biochemist named Kary Mullis. Mullis was doing research in the early 1980s at a biotechnology company in California when he initially thought of the idea regarding PCR. PCR was developed by a combination of several techniques already in existence; the synthesis of oligonucleotides and their utilization to synthesize new copies of DNA that were specific using DNA polymerases. Mullis…

Isolation and Antibiotic Resistance Testing of Delftia acidovorans from Organic Lettuce Pa Kou Thor Introduction Organic foods continue to be a health choice trend for many United States Americans as they are seen as the healthiest, natural and raw choice of food. However, there has been a growing concern of having antibiotic resistance bacteria existing on fresh organic produce. This combats the long-term thought that organic food, being free of chemicals and antibiotics, would not have…

Antibiotic sensitivity is the susceptibility of bacteria to antibiotics. Susceptibility can vary within bacteria of the same species due to different strains; with some strains being more resistant than others. Antimicrobial resistance is the ability of a microorganism to survive and multiply in the presence of an antimicrobial agent that would normally inhibit or kill this species of microorganism. Antibiotic susceptibility tests are done in order to determine, which specific antimicrobials…

similarity to 1/20 and 1/40 as displayed on the graphs. This might be due to collisions between the molecules. Since temperature plays a huge role by affecting the shape and activity of enzyme, it must be kept higher for the enzyme to not cause denaturation. During this lab, the enzyme was always kept in the ice-cold bath. Similarity, people put most fruits and vegetables in the fridge. Thus, putting cut open potatoes in a refrigerator would help keep PPO active and prevent enzymatic browning.…

The main objective of the experiment is to study and localize the fluorescent tags on specific chimeric proteins in a particular cell. The localization of these tags will generate a detailed understanding of the cytoskeleton in the cell, and the main function of using the Enhanced Yellow Fluorescent Protein. With the help of EYFP, the location of the proteins, and the molecular masses of the chimeric proteins should be really easy (Cannons, A. 2006). Consequently to better understand this…

"gelatinous". Gelatin is an irreversibly hydrolyzed form of collagen, wherein the hydrolysis results in the reduction of protein fibrils into smaller peptides, which will have broad molecular weight ranges associated with physical and chemical methods of denaturation, based on the process of hydrolysis. It is a biocompatible polymer and used for many biomedical applications including wound dressing with hemostatic…

Pomegranate consists of phenol compounds that are acidic and thus it has hydrogen ions that contribute to an increase in charged ions around the protein. These charged ions interact with the structural bonds in the protein structure and cause protein denaturation. Thus, it explains the decrease in protein percentage in the coconut pomegranate spread. Common coconut spread protein content varies from 3 to 8%. Although the protein content is lowered, it still exceeds the minimum value set by…

Quavian Belton Biochemistry I Dr.Nicholas Panasik October 6, 2015 PCR Optimization Abstract A Polymerase Chain Reaction (PCR) is a reaction that is set to catalyze the amplification of a copy of a piece of DNA. The aim of this lab was to optimize a PCR reaction by finding out the optimum concentration of MgCl2 needed. Six reactions were set up four with varying concentration MgCl2 and two primer control groups. The volumes and concentrations of each component of the PCR…

Lactate dehydrogenase was assayed according to the method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5.…

Cancer is a disease affecting the world and the rise in treatments with drugs is just one way of delaying the process of the disease as well as improving the quality of life for the patient. Henceforth Amsacrine is one to watch out for due to its specific mode of action. Reference. Amsacrine has an acridine chromophore with an amine at the 9 position (fig 1.) used in the treatment of some types of lymphoma or acute adult leukaemia [5]. It can also be known as Amsidine or m-AMSA [1]. Lymphoma…