Catalase

Enzyme Post- lab assignment The purpose of this lab is to see the enzyme activity. We have known that biological chemical reactions are catalyzed by enzymes, so in the experiment 4.1 we tested the properties of catalase, and its presence in potato and sheep blood. The result was we could see the oxygen gas- bubbling by catalase’s presence. Not only that, enzymes are known as reusable, but they are not altered by their reaction with any substrate. In experiment 4.2, we could see that enzymes…

away and bacteria will appear pink. Catalase test is an enzyme capable of decomposing hydrogen peroxide (H2O2) into water (H2O) and oxygen (O) 2H2O2 + 2H2O + O2 → catalase. Drop 1 small drop of 3% H2O2 cover up the bacteria and observed the formation of bubbles. (1) If the result give strong bubbles immediately after 1 drop, then this bacterium can be considering as catalase (+). In contract, if after drop H2O2 and bubbles not form then the bacteria is catalase (-). The main ingredients of MSA…

strings of growth within the anaerobic zone. Due to this observation it was interpreted that the bacterium was indeed a facultative anaerobe. Finally through the catalase test, due to the fact that the organism caused the hydrogen peroxide to bubble immediately, it was interpreted to have the enzyme catalase and therefore is said to be catalase positive. Test Purpose Observations Result Gram Staining The purpose of this test is to determine the shape as well as the nature of the bacteria’s…

free amino acid was calculated by plotting standard glycine. Catalase Catalase activity was measured by the method of Aebi, 1974[12]. 0.1 ml supernatant was addedtocuvette containing 1.9 ml of 50 mM phosphate buffer (pH 7.0). Reaction was started by the addition of 1.0 ml of freshly prepared 30 mM H2O2. The rate of decomposition of H2O2 was measured spectrophotometrically from changes in absorbance at 240 nm. Activity of catalase was calculated by extinction coefficient and expressed as…

PART A. Hypothesis: The Effect of Catalase Concentration It was hypothesized that if different number of disks would relate to the rate of the reaction produced then increased number of disks would result in increased rate of reaction. This occurs because an increased enzyme presence allows the substrate to rapidly change into a product providing all other factors such as substrate concentration, pH, ionic concentration, and temperature remain unchanged or fixed. ("What Is the Effect of…

identify an unknown bacterium by various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test, Motility, Triple Sugar test, Carbohydrate Fermentation test, and the IMViC test. Especially, the Gram stain differentiates between two large groups of microorganisms. Plan 03-05-16 The Unknown I.D. Project…

and after incubation, observed no clearing thus determining both samples to be gamma hemolytic. We gram stained each sample to confirm them being gram-positive, and then performed a catalase test; a positive result is indicated by the presence of bubbles, O2 gas, being released, and we observed both samples to be catalase positive. Next, we perform a coagulase test because a positive result may mean that the patient is dealing with a Staphylococcus aureus infection, as this species typically…

yeast catalase were incubated at varying temperatures to determine when decreased enzyme activity occurred. Hypothesis included sudden loss of function at temperatures above 45°C. Methods: Procedures outlined by Dr. John Choinski and Dr. Steven Karafit in Principles of Biology 1: lab notes manual, were followed (Choinski & Karafit, 2016). Temperatures tested included 25, 32, 39, 46 and 53 °C. Each sample was held for 2 minutes at each temperature. Results: Incubation of yeast catalase…

Introduction: Bacteria are very diverse and are part of the prokaryotic domain. Some bacteria can be harmful, but others can also be beneficial in many ways. There are bacteria everywhere on Earth; places that you would think would have bacteria, like your gastrointestinal tract or our skin (Wenner, 2007). Although bacteria are small in size and are a simple cell organism, it is essential to life. There are different ways in how to distinguish and classify bacteria. One way is you can look…

work on creating substances until the reaction is complete or the particular enzyme is inactivated, which either means being destroyed & recycled or paused, for the essential enzymes needed on a regular basis. An example of a necessary enzyme is catalase, better known as peroxidase, which catalyses or decomposes the reaction H2O2 (hydrogen peroxide). This enzyme splits hydrogen peroxide, an agent which interferes negatively with others like melanin (provides pigment for hair and skin), and…