Assay

METHOD DEVELOPMENT: To develop a suitable and robust LC assay method for losartan potassium and amlodipine, different mobile phases were employed to achieve the best separation and resolution. The method development was started with Aligent,Zorbax column (250 mmx4.6 mm I.D; particle size 5µm) with the following…

 Measurements The LifeShirt, a lightweight shirt made by VivoMetrics with built-in sensors capable of measuring respirational activity, heart rate, and subject posture and activity levels, among other things, was used to measure heart rate (HR) and RSA. RSA is used to determine automatic “cardiac control” by the brain in a subject and is a measurement of changes in heart rate during a respiratory period. HR and RSA were measured every five minutes between the introduction of the subjects to the…

mitochondrial fractions were then created to serve as the enzyme-rich solutions the reagent would act upon. To determine the protein concentration of the mitochondrial fraction, the Bradford assay was implemented; the result will later be used in the calculation of the specific activity of SDH. Ultimately, the SDH assay provided the data to either support or negate the hypothesis about malonate’s effect on SDH activity. This procedure involved testing tubes containing: potassium phosphate…

Chemicals: Folin-Ciocalteu reagent, sodium carbonate, gallic acid, trifluoroacetic and sulphuric acids, Trolox (6-hydroxy -2, 5, 7, 8- tetramethyl chromane-2-carboxylic acid), ABTS (2, 2'-azinobis (2-methylpropionamidine) dihydrochloride), 2′, 7′-Dichlorodihydrofluorescein diacetate (H2DCFDA), dexamethasone (DEX), and lipopolysaccharide (LPS) were purchased from Sigma Inc. (St. Louis, MO). Catechin, epicatechin, procyanidin B1 and B2 were purchased from Chromadex (Irvine, CA). Dulbecco’s…

endometrial surface epithelial cells. These cells were then injected with the 200 nM DJ-1 SMARTpool siRNA, which specifically targeted the DJ-1 gene or the non-silencing control. Protein extraction and immunoblotting were then done to prepare the adhesion assay. The cells were lysed and the proteins were eluted through boiling in 3X SDS sample buffer and then the amino concentration was calculated. The proteins were placed in a nitrocellulose membrane, in which they were then ready for…

Introduction: High-sensitivity cardiac troponin assays have provided a significant contribution for the early diagnosis of cardiovascular events due to their ability to detect lower concentrations of troponin levels more rapidly. There is significant evidence about the association between the absolute value of elevated cardiac troponin levels with the prognosis of patients with chest pain (3). However, it is well-known that elevated cardiac troponin levels may occur in situations other than…

to confluence. Then the cells will be treated with butyl hydroperoxide with and without n-acetylcysteine through standard injection ports. Assays will be initiated by replacing the growth medium with XF assay medium, which is formulated with unbuffered Dulbecco's modified Eagle's medium, 2 mM sodium pyruvate and 25 mM glucose, pH 7.4. The mitochondrial assays will include oligomycin (to inhibit ATP synthase activity), tri-fluorocarbonylcyanide phenylhydrazone (FCCP) (to uncouple mitochondrial…

2.1 Ageratum conyzoides Ageratum is derived from Greek word, which is “a geras” meaning non-aging, referring to the longevity of the flowers or the whole plant. The word “conyzoides” is derived from word “konyz” meaning resembles (M.X et al., 2016). Ageratum conyzoides is belongs to the Asteraceae family. Plant species in this family are herbaceous plants which trees and shrubs are rare. This plant is very common in West Africa and Asia. It has softly hairy stems and leaves, erect and branched…

The hypothesis of the experiment was that the molecular weight of N-acetyl-β-D-hexosaminidase would be around 28,000 kDa but to determine the molecular weight the concept of SDS-PAGE had to be understood. The movement of any charged particle through an electric field is determined by its net charge, its molecular radius and the magnitude of the applied field (7). Being that natively folded proteins are not molecular weight dependent, the proteins would be traveling at different speeds in an…

A colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich Chemical Co.) assay was performed to measure the cell viability. Briefly, RAW 264.7 cells were treated with different concentrations of spermidine for 24 h or pretreated with spermidine for 1 h before stimulation with LPS for 24 h. After incubation, the medium was discarded, and MTT solution (5 mg/mL in phosphate-buffered saline, PBS) was added to each well and incubated for another 3 h at 37 °C. The…