Agar plate

control. divided the plates into three section. The sections were labeled TRY 138, TRY 140, TRY 143. TRY 138 has no modifications to the FLO protein. TRY 140 has a decreased amount of FLO proteins while TRY 143 has an increase in the amount of FLO protein. After the sections were labeled, one member of the group used a sterile toothpick and used that to gently touch the center of the yeast growth of one of the sample strains. The same member then touched the center of the agar surface in the…

ESKAPE pathogens are six organisms that bring a huge threat to human, because they are drug-resistance. Scientists have not found antibiotics that are effective since the pathogens are multi-drug resistance. Multi-drug resistance is top three on global public health that are mostly caused by nosocomial infections (hospital-acquired infection caused by bacteria, fungal and viral, parasites and other pathogens). The six pathogens are Enterococcus faecium, Staphylococcus aureus, Klebsiella…

Since the beginning of time, Staphylococcus, also known as staph infection, has (Khan, 2017), but there was not an official discovery of what it was until much later. In the year of 1880, a surgeon discovered the bacterial infection in a patient after a surgery (Mandal, 2012). A staph infection is an infection caused by a group of Staphylococcus bacteria that can cause many different conditions and diseases. Believe it or not, the Staphylococcus bacteria is found in the normal flora of the body…

First step is to determine the total mass of plasmid DNA used. Then, calculate the total volume of cell suspension prepared. Next, calculate the fraction of the total cell suspension that was spread on the plate. Next, determine the mass of plasmid DNA in cell suspension (total mass of plasmid DNA x fraction spread). Final and fifth step is to determine the number of colonies per μg of plasmid DNA (number of colonies observed/step 4) .Below are the example…

compared. One sample was obtained from a hot, dry environment while the other was obtained in an area of dense foliage and animal traffic near a large pond. These samples were tested using nutrient agar for “simple” soil bacteria, glycerol yeast extract agar for Actinobacteria, and Sabouraud dextrose agar with antibiotic for fungi. For the first step, dilutions were prepared.…

dilutions is made from water sample collected, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. Dilutions are designed in order to make the calculation of the number of cells/ml in the original sample easier. The most common dilutions are 1/10, 1/100, and 1/1000. After incubation, agar plates with 30-300 colonies are counted. Greater than 300 colonies on the agar plate and less than 30 leads to a high degree of error. This range is chosen…

Introduction The cultivation and enumeration of a bacteriophage is a vital component of microbiology. Bacteriophages are used for a number of scientific applications, including the decontamination of food products, human therapy, and much more, thus it is critical to determine the concentration of the bacteriophage in a solution. Doing so allows scientists to determine bacteriophage potency, side effects, and minimal effective doses for clinical applications (Andersen et al., 2011). This…

existence of DNase entails certain strains/species of bacteria and may be utilized for typing. A DNase assessment is determined by cultivation on an agar plate containing DNA. If the bacterium contains DNase and the bacterium grows overnight the DNA will have the ability to hydrolyze into the constituting nucleotide. HCI is diluted and poured into the plate. A streak appears…

Staphylococcus aureus within the throat. In the collection and isolation of the mixed culture from the nose and throat there were seven different test that aided in conformation of these two samples, which was the MSA fermentation plates, the beta hemolysis of the blood and DNA agar plate, the coagulase test, Antimicrobial substibility, the characteristics that were viewed under the microscope, and the catalase test. Out of these test, the determining factor in detecting positive or negative…

Fifteen minutes of incubation was allowed for the infection to take place. After the fifteen minutes, 3 mL of agar was added and mixed into each tube. TSA plates were labeled with 10-4, 10-5, and 10-6. The mixture of agar and the dilutions were added to the corresponding plate. Each plate was sealed and moved in a circular motion on the lab counter to spread agar evenly. Finally, each plate was incubated right side up at…