Agar plate

It was used to make the nutrient broth. Then 2g of agar was added to the nutrients and it was dissolved in 100 mL distilled water and used as nutrient agar. The medium was sterilized. Maintenance of Bacterial Culture Bacillus cereus and Pseudomonas aeruginosa were grown on nutrient agar plates and incubated at 370C for 24 hours. The bacterial culture was maintained at 40C and sub cultured routinely to ensure viability of…

-Antibiotic disk of gentamycin, novobiacin and penicillin -Nutrient agar in petri plate. -Metric ruler, markers and plastic tweezers. -Tea candle light, sterile swab, rubbing alcohol and bleach -Other includes personal protective equipment like an apron, gloves, and safety goggles. Kirby Bauer diffusion is widely used test for…

have grown resistant to certain antibiotics and in this experiment, we tested how S. marcescens reacted to 6 different antibiotics. My colleagues and I swabbed a mixture of nutrient broth and Serratia marcescens onto an agar plate containing 6 antibiotic tablets and let the plate sit for a week. We then measured each tablet's zone of inhibition (ZOI) and pooled our data together. It was determined that the bacteria were most resistant to Ampicillin with an average ZOI of 7.6 mm, and least…

and Enterobacter aerogenes by the streak-plate method, which can be seen in Table 2. As demonstrated in Table 2, none of the antibiotic producers were found to be resistant against Enterococcus faecalis or Pseudomonas putida. Table 2 also showed isolates 3 and 4 were resistant to Acinetobacter baylyi, isolate 12 was resistant to Escherichia coli, and isolates 12 and 16 were resistant to Enterobacter aerogenes.…

Next, on the lid of each plate, you should draw four equal quadrants and label them c (control), 1, 2 and 3, as in the example photo below. Be sure to make note of which antibiotic 1, 2 and 3 correspond with before moving on and repeating these steps on the second agar dish. Following this, on the side of each agar plate, make a label indicting your teacher name, period number, lab group and bacteria sample, this will allow…

CHAPTER 3 3.0 Introduction To establish the differences in consumer acceptance, the pork liver polony sample was compared against the Colcom standard polony. Experiments were conducted by manipulating the independent variable, adding pork liver in two of the polony treatments, and observing differences between the test samples and the control sample. Analyses were used to quantify and describe the differences in shelf life, sensory evaluation scores, microbiological analysis and proximate…

The Lysine Iron Agar (LIA) black cap tube will be used to test decarbonation lysine, if positive for decarbonation it will have a purple slant and if negative it will be a darker red color. The LIA tube will also be used to test decarboxylate lysine, which will help us…

World Book Student defines genetic engineering as “techniques that alter the genes (hereditary material) or combination of genes in an organism… Beginning in the 1970’s, scientists developed ways to reintroduce individual genes into cells or into plants, animals, or other organisms” (Rubenstein, 2015). Genetic engineering is a controversial subject not only in science but also in popular culture. Some research, such as stem cell research, has led to serious questions concerning ethics. Other…

thaliana seeds inoculated on an agar plate containing 1mM of phosphate (NaH2PO4), while in the experimental (low phosphate) group, 42 A. thaliana seeds were inoculated on a 1 μM phosphate agar plate (Biology 108 Lab Manual 2015). There were 6 agar plates (Class groups) within each treatment group. Using sterile technique with a pasteur pipette, 1 seed per agar plate grid-line was inoculated for a total of 7 seeds per treatment. The agar plates were sealed, labeled, and incubated at room…

Germs are everywhere. Fortunately, most pose no risks to people. And those that do cause disease usually can be killed with antibiotic drugs. Sometimes, however, harmful bacteria evolve ways to “laugh at” antibiotics — survive as if the poisons were not even there. This so-called drug resistance make infections hard, if not impossible, to treat. How bacteria learn to resist killer drugs normally is invisible. But scientists have just unveiled a new tool that lets them watch it happen, right…