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9 Cards in this Set
- Front
- Back
•Know the methods to quantitate and
fractionate proteins particularly protein electrophoresis. |
•turbidimetry,
•colorimetry, •absorption spectrophotometry, •dye binding, •column chromatography, •electrophoresis, •and immunoassays. |
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The major proteins in plasma that contribute to the electrophoretic pattern
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albumin, α1-antitrypsin, α2-macroglobulin, haptoglobin, β-lipoprotein, transferrin, complement C3, fibrinogen, and
immunoglobulins. |
|
how are ceruloplasmin, C-reactive
protein, prealbumin, and protease inhibitors quantified? |
immunoassays.
|
|
Hereditary deficiency of what protein causes emphysema?
|
α1-antitrypsin
|
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The major clinical use of serum and urine protein electrophoresis is to
screen for ______ |
monoclonal gammopathies.
|
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The most frequently performed measurement, that
for total protein, is usually performed on serum, which has no ______ and no ________ that may slightly dilute proteins in plasma. |
fibrinogen
anticoagulant |
|
__________ separates the globulins from
albumin |
•Protein electrophoresis
|
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(1) chronic inflammation with decreased albumin and increased γ-
globulins; (2) acute inflammation with increased α2-fraction (haptoglobin) and decreased C3 due to activation and consumption of complement; (3) inanition post-spinal cord injury with hypoproteinemia of several fractions. |
?
|
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Describe the Serum and urine protein electrophoretic patterns in a patient with multiple myeloma.
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Serum demonstrates a predominance of the larger
complete immunoglobulin, while the urine has a large amount of the smaller-sized light chains with only a small amount of the whole immunoglobulin. |