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9 Cards in this Set

  • Front
  • Back
•Know the methods to quantitate and
fractionate proteins particularly protein
electrophoresis.
•turbidimetry,
•colorimetry,
•absorption spectrophotometry,
•dye binding,
•column chromatography,
•electrophoresis,
•and immunoassays.
The major proteins in plasma that contribute to the electrophoretic pattern
albumin, α1-antitrypsin, α2-macroglobulin, haptoglobin, β-lipoprotein, transferrin, complement C3, fibrinogen, and
immunoglobulins.
how are ceruloplasmin, C-reactive
protein, prealbumin, and protease inhibitors quantified?
immunoassays.
Hereditary deficiency of what protein causes emphysema?
α1-antitrypsin
The major clinical use of serum and urine protein electrophoresis is to
screen for ______
monoclonal gammopathies.
The most frequently performed measurement, that
for total protein, is usually performed on serum,
which has no ______ and no ________ that
may slightly dilute proteins in plasma.
fibrinogen

anticoagulant
__________ separates the globulins from
albumin
•Protein electrophoresis
(1) chronic inflammation with decreased albumin and increased γ-
globulins;
(2) acute inflammation with increased α2-fraction (haptoglobin) and decreased C3
due to activation and consumption of complement;
(3) inanition post-spinal cord injury with
hypoproteinemia of several fractions.
?
Describe the Serum and urine protein electrophoretic patterns in a patient with multiple myeloma.
Serum demonstrates a predominance of the larger
complete immunoglobulin, while the urine has a large amount of the smaller-sized light chains with only a small amount of the whole
immunoglobulin.