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14 Cards in this Set
- Front
- Back
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Why can't some bacteria be grown in pure culture?
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Some microorganisms need very specific conditions to survive.
It is difficult to recreate those conditions in a lab. Others only survive with certain neighbors. Ex- One bacterium releases methane while other consumes it |
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Limitations to Kosh's Postulates
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Limited to culturable only microbes
Some symptoms of infections are ambiguous Examples of exceptions: syphilis, leprosy, all viral diseases, prions |
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Three Major Characteristics for Bacterial Classification
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1. Macroscoscopic colony morpholohy (Naked Eye Evaluation)
2.Microscopic Morphological characteristics 3. Different staining characteristics |
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Macroscopic Colony Morphology
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Smooth/rough
Opaque/Transluscent Pigment |
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Microscopic Morphological characteristics
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Cell Shapes & Arrangements
Presense or absence of endospores, flagella Location of endospores |
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Different Staining Characteristics
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Gram Pos- Pink
Gram Neg- Purple Nature of dif. is cell wall structure |
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glycocalyx
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gummy external coat, polypeptides, polysaccarides or both,
capsule- organized & firmly attached, slime layer- unorganized and loosly attached improves attachment & food storage |
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spirochete
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spiral shaped bacterium w/ axial filaments
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staphylo
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grapelike, irregular structure
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strepto
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chains
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Primary Structure
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Unique sequence in which amino acids are linked together to form a a genetically determined polypeptide chain
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Seconday Structure
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Localized, repetitious twisting or folding of the polypeptide chain
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Tertiary Structure
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Overall 3D structure of polypeptide chain folded helix and pleated sheets
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Quaternary Structure
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Consists of an aggregation of two or more individual polypeptide chains (subunits) that operate as a single function
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