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27 Cards in this Set
- Front
- Back
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the of the clinical lab is to provide you with information, the information you need from the lab is:
1. 2. |
1. identify the presence or absence of suspected pathogen
2. antibiotic sensitivity profile of suspected pathogen |
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from where does sputum come:
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the lungs
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what types of collected specimens for the clinical lab should be sterile but are usually contaminated:
1. 2. |
urine
sputum |
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..., ... and ... are all normally sterile and any bacteria found in these samples are potential pathogens
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Blood
CSF tissue samples |
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what is the major drawback to identifying bacteria by culture:
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takes 2 to 3 days to get results
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what is specificity:
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measure of how often the test or method gives false positive results
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what is sensitivity:
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measure of how often the test or method gives false negative results
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acid fast stains what organism discussed in class:
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Mycobacterium tuberculosis
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if a sample of urethral discharge from a male patient shows Gram-negative diplococci, ... is the most likely pathogen
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Neisseria gonorrhoeae
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a patient with a persistent cough, night sweats, fever and shows an acid-fast bacillus in their sputum, the organism is most likely ...
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Mycobacterium tuberculosis
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... staining is a very specific method of identifying an organism
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Fluorescent antibody
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what would you use to check for Helicobacter pylori in a stool sample:
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enzyme immunoassay (EIA) test
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the following describes what organism:
This organism causes ulcers and can be grown in culture, however it requires a gastric biopsy to get a tissue sample. An alternative is to use antibodies made against it to detect the presence of the organism in stool samples |
Helicobacter pylori
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what does the above picture depict:
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enzyme immunoassay (EIA) test
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Chlamydia trachomatis must be grown in tissue culture, but since it has a specific DNA sequence, what kind of kit has been developed to detect it:
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PCR
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what is an indirect method of determining if a person has an infection with a given pathogen
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serological tests to see if the patient has antibodies
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what is the organism that causes Rocky Mountain spotted fever:
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Rickettsia rickettsii
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what is the problem with serology:
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it takes several days to weeks for antibodies titers to rise high enough to be sure an active infection is taking place
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the following describes what organism:
this organism is part of the normal intestinal flora and if a person that carries this strain is given antibiotics that wipe out a large portion of the bacteria, it will overgrow and produce toxin |
Clostridium difficile
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how would you test to see if Clostridium difficile was producing toxin:
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test fecal specimen with antibodies against the toxin
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The Sanford Guide to Antimicrobial Therapy provides recommendations for antibiotics to use until the ... report comes back.
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sensitivity
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resistance to the antibiotic vancomycin evolved in ... then the genes for vancomycin resistance spread to ...
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Enterococcus
Staphylococcus aureus |
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are all strains of Staphylococcus aureus resistant to vancomycin:
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no
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what is the name of the organism that causes tuberculosis:
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Mycobacterium tuberculosis
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what does the following describe:
specimen is plated on agar and grown overnight then tests are performed to identify the likely pathogen. a suspension is spread on an agar plate and paper disks containing different antibiotics are placed on the agar surface then incubated overnight. |
Antibiotic Disk Diffusion Tests
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the lowest concentration of an antibiotic that will prevent growth of the bacterium:
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minimum inhibitory concentration (MIC)
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what would be a faster method to determine the presence of antibiotic resistance other than antibiotic disk diffusion tests:
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PCR
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