Week 5 Monoclonal Ab

Front 1

What is the humoral immune response? [LO]

Back 1

[LO]

Antibodies produced by B cells (lymphocytes) destroy extracellular pathogens and prevent the spread of intracellular infections.
•‘antibody-mediated immunity’
•(as opposed to the ‘cell-mediated immune response’ effected by T cells (lymphocytes))

Front 2

•Describe the general process of B cell development from the bone marrow to plasma cell or memory B cell.
[LO]

Back 2

[LO]
- Lymph node
- paracortex where it is activated by T cell (CD4) or Ag/dendricyte
- dark zone germinal centre where B cell maturation and differentiation occurs
- Light zone accepted as memory cells or plasma cells

Front 3

Understand the link between myeloma and its properties used for monoclonal antibody production. [LO]

Back 3

Properties of myeloma cells are used to produce large quantities of monoclonal antibodies
Plasma cell = long-lived & ability to continuously secrete antibody
Fusion of plasma cell + spleen cell = hybridoma

Front 4

•Recognise the features of myeloma as a disease.
[LO]

Back 4

ABRH
EIU
10%

Front 5

•Describe the process of monoclonal antibody production and the role of key reagents. [LO]

Back 5

[LO]
1. inject with Ag
2. remove spleen HGPRT neg
3. MM with HGPRT neg cells = hybridomas
4. culture in HAT medium
5. test supernatant for Ab
6. Expland positive clones
7. Harvest in vitro or in vivo

Front 6

What is PEG?

Back 6

polyethylene glycol and assists the fusion of spleen cells and myeloma cells to form the hybridoma

Front 7

what is HAT?

Back 7

hypoxanthine aminopterin thymidine