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7 Cards in this Set
- Front
- Back
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Monoclonal antibodies |
Identical immunoglobulin molecules formed by 1 clone of plasma cells. Produced for specific antigen. Makes tests highly sensitive |
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Enzyme linked immunoabsorbent assay (ELISA) |
Hella common in clinic. Uses monoclonal antibodies. Viruses bacteria parasites hormones enzymes etc. |
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Direct ELISA |
Monoclonal antibody attached to well/membrane/ sample added and if antigen present it binds to the antibody, then there is a wash step and a dye changes the colour for a positive result |
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Indirect ELISA |
Antigen is stuck to well not antibody |
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Competitive ELISA |
Uses monoclonal antibodies and enzymatically labelled antigen. Patients antigens compete with tests antigens to bind to the antibody. Intensity of colour produced varies with the concentration of patient antigen |
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Latex agglutination |
Uses small latex particles coated in antigen - suspended in water. Antibody-antigen complex forms resulting in agglutination or clumping. Sample is positive |
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Rapid immunomigration |
Conjugated antibodies present, patient sample is added. If antigens present they bind to antibodies and migrate along the membrane of test cassette. |
