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26 Cards in this Set
- Front
- Back
- 3rd side (hint)
Tests performed on donors and recipients of kidney and BM transplants
How many? |
3
List |
Serological test Cellular test Molecular methods |
|
Serological test Cellular test Molecular methods
Tests performed on ____ and _____ of kidney and BM transplants |
Tests performed on donors and recipients of ____ and _____ transplants
List |
BM kidney
Serological, cellular test, molecular methods |
|
Serological test
Principle |
Use ab to identify
HLA antigen on patient cells Antibodies in patient serum
What test it is? how many examples there are? |
Serological test
3 |
|
3 examples of serological test |
ELISA Microcytotoxicity test Cross match test |
|
|
Cellular test
Principle |
Detect T cell response to HLA Ags on grafts
How many examples there are? |
2 |
|
2 examples of cellular test |
MLR MLC
Full name |
Mixed lymphocyte reaction Mixed lymphocyte culture |
|
Molecular test
Principles |
Identify HLA alleles
Examples |
PCR |
|
Serological test
ELISA _______________ Cross-match test |
Microcytotoxicity test |
|
|
Microcytotoxicity test
Aka |
Terasaki method
test for? |
HLA A, B, C, DR Ags |
|
Microcytotoxicity test
Test for? |
HLA A, B, C, DR ags
They are known as? |
SD Ags |
|
Microcytotoxicity test
Steps |
1. Obtain a well that contains antiserum against different HLA Ags in each well.
2. Add peripheral blood to each well For HLA A, B, C, add mononuclear cell For HLA DR, add purified B cells
3. Add reagent complement (from rabbit serum) Wells that have Ab to HLA ag will have cell lysis
4. Add viability stain Dead cells: permeability is altered, colored Live cells: colorless |
|
|
Microcytotoxicity test
How cells are examined? |
under phase contrast microscope |
|
|
Microcytotoxicity test
Positive result for HLA antigen |
50% of cells are pink |
|
|
Cross match test
Application |
Detect pre-formed Abs in recipient against donor's HLA abs
Purpose |
prevent hyperacute rejection |
|
Cross match test
procedure |
similar to Microcytotoxicity test
1. Serum from recipient + Peripheral blood (mononuclear cells)from donor (not patients) + C
2. Incubate in microtiter plate
3. Add viability stain
Interpret the results?
|
+: bad donor -: good donor |
|
MLC
aka |
Mixed lymphocyte reaction |
|
|
MLC/MLR
What does it detect? |
Differences between HLA-D Ags |
|
|
MLC procedure |
Lymphocytes from donor is mixed with Lymphocytes from recipient in a microtiter plate
If HLA-D from donor is different from recipient, recipient lymphocytes will proliferate
it is measured by 3-H, a radioactive dye that is incorporated into DNA |
3-H: thymine |
|
MLC
Limitations |
Labor intensive
How long it takes? Not applicable to? |
6 months
Cadaver |
|
Molecular methods
List |
PCR SOOP Sequence
Function |
PCR: Amplifies HLA gene SOOP: identify HLA allele by binding to HLA gene Sequence: HLA gene |
|
Immunosuppressive agents
List |
1. Anti-proliferative agents 2. Cortiosteroids 3. Calcineurine inhibitors 4. Anti-thymcyte globulin 5. Monoclonal antibodies |
|
|
Immunosuppresive agents
1. ______________ 2. Cortiosteroids 3. Calcineurine inhibitors 4. Anti-thymcyte globulin 5. Monoclonal antibodies |
Anti-proliferative agents
Example: Mycophenolate Mofetil, Azathioprine, Sirolimus
Mechanism |
Blocks DNA synthesis and therefore lymphocyte proliferation |
|
immunosuppressive agents
1. Anti-proliferative agents 2. ______________ 3. Calcineurine inhibitors 4. Anti-thymcyte globulin 5. Monoclonal antibodies |
Corticosteroids
Example: Prednisone, Methylprednisolone
Mechanism |
Block cytokine production by T and M cells
Antiinflammation |
|
Imunosuppressive agents
1. Antiproliferative agents 2. Cortiosterioids 3. _______________ 4. Anti-thromcyte globulin 5. Monoclonal antibodies |
Calcineurine inhibitors
Example: Cyclosporin A, Tacrolimus
Mechanism |
Decrease cytokine production |
|
Immunosuppressive agents
1. Anti-proliferative agents 2. Cortiosteriods 3. Calcineurine ihibitors 4. ___________________ 5. Monoclonal antibodies |
Anti-thromcyte globulin
Mechanism |
Destroy T cells |
|
Immunosuppressive agents
1. Anti-proliferative agents 2. Cortiosteriods 3. Calcineurine inhibitors 4. Anti-thromcyte globulin 5. _________________ |
Monoclonal antibodies
Examples: to CD3, IL2 receptor, adhesion molecules, cytokines |
Inhibits various steps of the immune response |