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20 Cards in this Set
- Front
- Back
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Donated products have to be tested for (allogeneic)
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ABO group
Rh type syphilis HbsAg HIV NAT HCV NAT anti-HIV-1 anti-HIV-2 anti-HBc anti-HCV anti-HTLV-I/II |
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Donated units require serological testing for syphilis; the spirochete survives in the refrigerated units?
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- unknown how long the spirochete survives at refrigerated temp in a naturally infected unit
- studies show that survival at 4C is dependent on concentration |
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confirmatory testing for HIV
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Western blot (most common confirmatory test for EIA-reactive anti-HIV tests)
- most infected people have Ab to almost all of the various viral gene products: 1. gag proteins: p17, p24, p55 2. pol proteins: p31, p51, p66 3. env glycoproteins: gp41, gp120. gp 160 Result: +, -, indeterminate +: reactive w/ at least 2 of the following - p24 core protein - gp41 transmembrane protein - gp120/160 external protein and external precursor protein |
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an immunofluorescence assay (IFA) can serve as an alternate confirmatory test for + anti-HIV EIA?
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yes
- test involves HIV infected cells |
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How is a + anti-HTLV EIA confirmed?
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Not Western blotting!
Use another manufacturer's anti-HTLV EIA!! - if the second EIA is + = confirmed positive - if the second EIA is - = the first is considered a false positive!! |
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anit-CMV testing is required by the AABB?
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NO!
- it is a commonly performed optional test |
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ISBT 128
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- a standardized labeling system that uses bar-coded labels that can be read by blood centers and transfusion services around the world
- internationally defined system using bar-code symbology called Code 128 |
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Whole blood contains __
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- all blood elements + anticoagulant-preservative in the collecting bag
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Minimum HCT of a whole blood unit?
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33%
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pRBC w/out AS (in CPD, CP2D, CDPA-1 alone) must have a HCT of ___
pRBCs w/ AS will have a HCT of ___ |
- 80% or less
- w/ AS, get a HCT between 55% - 65% (facilitates flow rates and eases administration) |
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amount of AS in a 450 ml pRBC unit vs 500 ml pRBC unit
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450 ml unit - 100 cc AS
500 ml unit - 110 cc AS |
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Additive solutions extend the RBC shelflife to ___
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42 days
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AS-1
AS-3 AS-5 |
AS-1
- Adsol AS-3 - Nutricel (no mannitol) (citrate present) AS-5 - Optisol |
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Shelflife
CPD CP2D CPDA-1 |
Shelflife
CPD - 21d CP2D - 21d CPDA-1 - 35d (adding the adenine) |
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platelet-rich-plasma (PRP) is prepared within __ hrs of collection of the whole blood unit
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8hrs
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whole blood derived platelets are prepared by __
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- soft spin (PRP)
- hard spin (platelet concentrate, FFP) |
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plasma can be prepared from a whole blood unit up to ___ days after the ___ date
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up to 5 days AFTER the expiration date
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There is a difference between Fresh Frozen Plasma (FFP) and Plasma Frozen Within 24 Hours after Phlebotomy
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FFP - plasma prepared within 8 hours of collection and frozen at -18C up to 12 months or at -65C up to 7 years
- Plasma Frozen Within 24 Hours after Phlebotomy - prepared between 8 and 24 hours after collection and frozen -18C for 12 months |
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FFP Thawed
Plasma Frozen Within 24 Hours after Phlebotomy Thawed |
both expire at 24hrs (with this label)
For FFP Thawed: If NOT transfused w/in 24hrs the lable MUST be modified to read "Thawed Plasma" |
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Thawed Plasma versus FFP Thawed
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"Thawed Plasma" is "FFP Thawed" that has been thawed for more than 24 hours
- expires 5 days after thawing - has less Facter V and VIII (esp VIII) compared to FFP |
