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8 Cards in this Set
- Front
- Back
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microscope
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an optical system for magnification and illumination
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Light Microscopy
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All give maximal useful magn. of 1000x-2000x*
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Bright -field microscopy-
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dark object on a bright background
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Magnification
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2 lens system, an objective lens and an ocular lens.
ocular X objective 3 or 4 objectives (scan=4x, high dry =40x{or 43x} Low power =10x oil objetive =100x |
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Illumination
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artificial light source also have a condenser lens system condenser lens concentrates or directs the light at the objective
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parfocal
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the image should remain in focus when objectives are changed
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resolving power {resolution}
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represents the ability to distinguish 2 points which are close together ;as distinct and separate.
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resolving power
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RP= wove length of light mumerical aperture of objective lens+ numerical aperture of condenser lens the smaller the rp the better the resolution
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