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6 Cards in this Set
- Front
- Back
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Gram stain
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1) Stain w/ crystal violet for 60 seconds
2) Wash w/ water and add iodine-KI for 60 seconds 3) Wash w/ water and decolorize w/ 95% alcohol 4) Counterstain w/ safranin for 30 seconds Gram + retain iodine-dye complex in the cell wall and turn purple-blue Gram - lose the iodine-dye complex after alcohol wash and retain safranin (red) |
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Staph epidermidis
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Gram positive cocci in clusters
Catalase positive Coagulase negative Facultative anaerobe Produces a mucoid substance and responsible for catheter-related infections |
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Staph saprophyticus
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Gram positive cocci in clusters
Catalase positive Coagulase negative Facultative anaerobe |
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Staph aureus
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Gram positive cocci in clusters
Catalase positive Coagulase positive Beta hemolytic Facultative anaerobe Exfoliant (scolded skin syndrome), Enterotoxin (food poisoning), Toxic shock syndrome Produces a mucoid substance and responsible for catheter-related infections, produces proteins that breaks down connective tissues and tissue proteins |
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Strep pyogenes
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Group A B hemolytic (only one sensitive to bacitracin)
Gram positive cocci in chains Catalase negative Facultative anaerobe responsible for the fever and rash associated w/ Scarlet fever certain strains block phagocytosis and cause necrotizing fasciitis (flesh-eating bacteria) |
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Strep agalactiae
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Group B B hemolytic (not as distinct as Group A)
Gram positive cocci in short chains or diplococci Catalase negative Facultative anaerobe Colonize vaginally |
