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155 Cards in this Set

  • Front
  • Back
PKU happens how often?
occurs in one of every 12,000 to 15,000 live births in the United States
To get PKU, both parents need to:
Both parents must be carriers in order for this gene to be inherited
The Enzyme Deficiency for PKU
The baby is lacking an enzyme called Phenylalnine Hydroxylase
PKU does:
PKU is a disease that affects the way the body processes food
As a result, phenylalanine builds up in the bloodstream and causes brain damage and mental retardation


Children with PKU cannot process a part of protein called phenylalanine.
How is the PKU test performed?
Blood is obtained by "heel stick" and collected on a special filter paper.
Capillary Punctures for infants for PKU
A heelstick is the preferred method
neonates and infants under 6 months of age
lateral portions of the heel
Obtaining specimen for PKU
Warm the infant’s foot for about 3 minutes with a moist towel.
Clean the infant’s heel with 70% isopropyl alcohol.
Allow the heel to air dry.
Puncture the infant’s heel with a sterile lancet having a tip no longer than 2.5mm.
Wipe away the first drop of blood with a sterile gauze.
Allow the second drop to form by spontaneous free flow of blood.
Elevate the infant’s foot above the body. Wipe the puncture area with a sterile gauze and apply a bandage as needed.
Term Words for the Infection Cycle
Droplets can travel up to 20 feet
Incubation- time from when the person was exposed to when they show symptoms
Susceptible-when a person or pt is more prone to get virus or bacteria infection
Most susceptible-Diabetic, cancer, aids pt, children, and geriatric pt, and those who are sick already
Direct contact- kissing, in the face
Indirect- touching something they touched, sharing eating utensils, touch contaminated tissue, then rubbing your mocous membranes (eyes, nose, mouth) with hand you touch with the infected item
The Infection Cycle
Microorganism pathogens- bacteria, viruses, fungus, parasites
Reservoir- known as location of infectious agent. Location can include human body or contaminated instrument. Ex- people, equipment, supplies, water, food, animals, and insects.
Means of escape-known as portal of exit, meaning how did it leave the reservoir. An infected agent must leave resevoir to infect another person, it leaves through, excretions, secretions, respiratory droplets, or blood.
Mode of transmission-from reservoir to the host. Happens through direct contact, airborne transmission, ingestion (meaning they ate it somehow) indirect contact, or insect bites.
Means of entry- enter thru broken skin, mucous membranes, respiratory tract (can breath it in ), GI tract, reproductive system.
Host susceptibility- people that have a compromised immune system (sick), children, elderly. The infectious agent must enter a person who is susceptible (weak) a susceptible host is not resistant, nor immune to the microorganism. * this is a big part of MIDTERM*
Growth Requirements for Microorganisms
Microorganisms must have asuitable environment for them to survive.
Ex-Fungi needs oxygen and moisture
Temp for most microorganisms to survive is 98.6F
Neutral ph balance is 6.0, anything above that indicates that microbial growth exists.
Infection Cycle: Infectious Agents
Ex of bacteria is tetanus
Non pathogenic bacteria live on skin and mucous membranes
Pathogenic bacteria always takes over non-pathogenic bacteria
Smallest of living microorganisms are viruses. They can only reproduce within a host. Once they attach they adapt
Common cold, herpes, and flu, are common viruses
Some ex are athletes foot, ring worm
Parasites- are lil bugs like the pin worm, and tape worm
Hep B Virus
Sym-Loss of appetite, nausea, vomiting, fever, abdominal pain, jaundice
100 times more infectious than HIV
Hep b can live on dried surfaces for one week.
HIV
Sym- night sweats, weight loss, swollen glands, most often there are no symptoms
Affects the immune system
Can lead to developing AIDS
Tuberculosis
May get Tb from Inhaling droplets from a cough or sneeze from an infected person
Natural Defense Mechanisms
Nose, mucous membranes, prostatic fluid, tears, and cervical mucous, all containing lysozyme, which splits the muramic acid linkage in bacterial cell walls
Respiratory tract has upper airway filters
Saliva has a special enzyme that protects us
The GI tract also has barriers that include the acid ph of the stomach
Control requirements for microorganisms
Be in good physical condition- healthy
Be in good emotional state
Have proper exercise weekly, and every other day
Have good nutrition
Have proper hygiene
Learn how to reduce stress in your life
Limit direct or indirect contact with infected people
Use universal precautions
Always practice infection control
Infection Control Sanitization
Wash and scrubb hands to remove body tissue and blood
When you sanitize instruments you wear utility gloves.
Infection Control Disinfect
Process by which disease producing microorganisms or pathogens are killed except spores
Pertains to a chemical or physical means of destroying bacteria
Germicide or bactericide
Used on objects not people
How are infectious agents transmitted?
From improper hand washing, run for 20 sec atleast, scrub, clean under nails, hot water
Also from instruments, werent clean enough
Also from body fluids, and needle sticks
Infection Control Sterilization
Destroys all life form including spores thru autoclave
Nosocomial mean?
Infection acquired in the hospital
Red Blood Cells are?
RBC- erothrocytes(what they are called)erothro(red) cytes(cell) the primary production of rbc’s is formed in bone marrow.
The term word Anatomy
Refers to the structure –size & shape
Adult person has approximately _Liters of blood
We can live with four and five liters of blood but our body holds 6 liters of blood. This is why we are allowed to donate blood
Functions of Blood
Transport oxygen to tissues
Transport waste products ( filter blood through the kidneys)
Transport nutrients to the whole body.
4 Parts of the Circulatory System
Heart
Blood vessels
Blood
Lymphatic system.
Structure of vessel walls
Walls of arteries and veins contain three distinct layers
Tunic intima
Tunica media
Tunica externa
Differences between arteries and veins
Arteries have thicker walls and have more smooth muscle and elastic fibers.
Arterial blood does:
Flows away from the heart. If you cut a persons arterey…artery blood spurts. Is bright red similar to the clor of this slide. Blood that is coming from vein is a lot darker because its oxygenated. Largest artery of the body is aorta
Arteries
Vasoconstriction-vessels decrease in the side of the lumen
Vessell is something that transport things.
Vasodilation- vessels increase in size.
Blood Vessels
Arteries, arterioles, veins, venules, and capillaries.
The bigger vessel is the arteries but the arteries can be smaller and their called arterioles.
Veins smallest part is muscle.
Veins
Collect blood from all tissues and organs and return it to the heart
Are classified according to size
_Large veins___________
_medium veins___________
___venules_________
Venous Valves
Venules and medium-sized veins contain valves
Prevent backflow of blood
Distribution of blood Pulmonary Circulation
*Pulmonary circulation is the flow of blood between the heart and lungs
*oxygen is inhaled into the lungs from the air we breath; it starts from our mouth/ nose to the trachea (wind pipe), to the bronchioles, which branch out to the lungs.
*At the bottom of our lungs we have little tiny sacs called alveoli's, which resemble grape like clusters, that are surrounded with microscopic capillaries that transport the oxygenated blood into our blood stream.
wash hands properly
Wet your hands with warm, running water and apply liquid Antibacterial soap Lather well! Rub your hands vigorously together for at least 20 to 30 seconds.
wash hands properly
Scrub all surfaces, including the backs of your hands, wrists, between your fingers and under your fingernails
Cellular components
Whats in blood is blood cells
Type 1-erythrocytes-RBC-red blood cells
Type2-leukocytes-WBC-white blood cells
Type 3-thrombocytes-platelets
Erythrocytes
They carry- oxygen and carbon dioxide
What protein- hemoglobin (combo of iron and protein that gives blood its red color)
What color-red
RBC lives for four months
Produced in bone marrow at a rate of one million per second, and at this same rate they wear out
Erythrocytes
4.5-5million RBC per cubic ml of blood
They are biconcave on both sides
This allows for flexibility so they may pass thru capillaries
They contain hemoglobin which helps O2 attach to cell
Leukocytes WBC’s normal range is ___________per cubic millimeter.
5,000-9,000 normal range
There are 5 main WBC’s:
Neutrophils
Basophils
Lymphocytes
Monocytes
Eosimophils
Leukocytes
1.Responsible for destroying foreign substances such as pathogens
2.Pass thru capillary walls to enter tissue
4.Leukocytes are round and white and lack hemoglobin
Thrombocytes
Known as platelets
Smallest in size compared to other cell’s-smallest of the blood cells
Looks like broken pieces
Platelets stick together at site of injury forming a platelet plug, promotes coagulation- promotes blood clotting
Normal range is 200,000-400,000 per cubic of blood
Liquid Component is
Liquid portion of whole blood
Made up of Plasma and Serum
Plasma
Plasma is a pale yellow fliud
Made mostly of water
Mixed with nutrients, some proteins, ions, gases, waste, fibrinogen
It assist’s with clotting of blood (fibrinogen)
Blood is
Red Blood Cells (Erythrocytes)
- White Blood Cells
(Leukocytes)
- Platelets
(Thrombocytes)
- Plasma
(Lymph)
COMMUNICABLE DISEASES
A communicable disease such as a cold, is a disease that spreads from person to person. These are diseases you can catch from someone or something else
To prevent these types keep immunizations up to date, and wash hands thoroughly and frequently, watch what you eat, and how you eat it.
Influenza
Caused by myxo virus
Affects resiratory tract
Direct and indirect
Inc-1-4 days
Sym- fever, chills, sore throat
PNEUMONIA
Caused by Pneumococcus bacterium
Inflammation of lungs
Inc- few hours after exposure
Sym- severe chills, fever, chest pain, difficulty breathing, blood stained sputum
Tetanus
Acute (comes fast) fatal infectious disease
Affects CNS
Referred to as lock jaw
Caused by bacillus clostridium tetani
Transmitted by puncture wounds
Happens with abrasions
Happens from laserations (cuts)
Inc-3-21 days
Sym- stiffness of jaw, stiffness of esophageal muscles, fever, and painful muscle spasms
Rabies
Viral disease transmitted in saliva of infected animals
Sym- aggressive behavior, thirst, fever, pain, extreme weakness, and hallucinations
Varicella Zoster
Chicken pox
Highly contagious
Direct contact and droplets from respiratory tract
Member of herpes virus family
Inc- few days to two weeks
Sym- highly piuritic rash, fever, headache, loss of appetite, general malaise (fatigue)
HEPATITIS A “INFECTIOUS HEPATITIS
Sym- similar to hep b
Vaccine Recommeded for high risk groups, those who travel to other countries
HEPATITIS B “SERUM HEPATITIS
Potentially fatal
Transmitted by contaminated serum via blood transfusions, and contaminated needle use.
Inc-14-50 days
Sym-above, and dark urine, fever
HEPATITIS C
Blood borne infectious viral diesease caused by Hep C virus infection can cause liver inflammation which is known as hepatitis
Asymptomatic- without symptom
But later the liver gets cirrhosis- which is fibrotic scarring of the liver,
And it can also cause liver cancer
Can also catch this from having sex with an infected person
Also being born to a mother with hep c
Sharing drug needles, or getting pricked by a needle in health care field.
BACTERIAL MENINGITIS
Highly contagious
Causes inflammation of the meninges of the brain and spinal cord
Causes serious long lasting effects to CNS
You can die within 24-48 hrs
Sym- high fever, chills, headache, vomiting
HIV
Primarily infects human immune system
Specifically our T cells
Phlebotomy
Means to draw blood
Hematology
Is the scientific study of blood and blood forming tissues. A hemotologist physician who specializes in the functions and disorders of the blood. Why is it acetic?(blood)
Blood is the life-maintaining fluid that circulates through the body's
Heart
*arteries
*veins
Capillaries
Allied health personnel, licensed and unlicensed health care professionals who withdraw
*physicians
Disease intervention specialist
*Medical Researchers
Nurses (lVN, RN, LPN)
*Physician Assistants
* Medical technologists
*Radiologic technologists
*EMT
*Military Medics
*Phlebotomists
* Medical Assistants
The purpose of phlebotomy
Is to obtain a blood sample for diagnostic testing
Removing blood
The process of removing blood from the veins date back to 1400 B.C. In the early 1800’s leeches were used ina procedure called blood letting.
In the early 1800's....
leeches_________ were used in a procedure called “____________bloodletting________”

Bloodletting was thought to rid the body of ____impurities_____ and evil
spirits
Leeches
Leeches were and still used for blood letting commonly used in europe. Technihian applies leeches to an infected area or they want inflammation to go down. Leeches fall off the body when their full so don’t pull off. Some people use it for inflammation in the knees.
Capillary Punctures
Do not retract, put in the thing looks like a pen……left hand side is a retractable though…general lancids are used for obtaining small samples of blood from the fingertip or heel.
Hematocrit
This is a test to determine red blood cell count. Prick a finger and learn how to fill up glass tubes and spin in a machine
Hematocrit
Centerfuse machine and glass tubes. U have to separate blood from fluids
Blood Glucose Test
Test to Measure the sugar in blood..sugar is called glucose and glucose is found in carbohydrate foods.
Venipunctures
The term for blood drawing…the word veni means vein and punctures is to puncture the vein.
Winged Infusion Set
A lot of people call these butterflies.
Erythrocyte Sedimentation Rate
This is a test..known as esr a test used to configure the amount of inflammation in the body. Test is usually for artherits, diabetics, cancer patients….inflammations can be sign of an infection
Urinalysis
Analysis of urine..considered a fast accurate and non invasive procedure…still have to wear protective equipment. Ayour liver, pancreas, blood, and kidneys is a mini physical of your body is a UA
Strep Test
This is quick in the office……where we test for strep throat…it can affect your heart and u can die
Mono Testing
Mono is known as the kissing disease.
Why a Blood Test is needed?
Diagnostic Purposes....examples?
Yearly Physicals
Septic (poison in the blood)
Inflammation
Hepatitis
Sex
Anemia (low iron)
Polycythemia (too many cells)
Cancer
Diabetes
High Cholesterol
STD’s
Autoimmune disorders (aids,)
Heart Problems
DNA
Blood Type (apositive, b negative)
Bleeding Disorder
Pregnancy
Kidney Function
Arthritis
AIDS/HIV
Liver function
Thyroid function
Centrifuge
Technicians use this machine to spin blood samples..not all samples are spun and some are spun longer or shorter than others.
Tourniquet Color Tubes
Tie off the blood flow- tourniquet(cant leave on for more than one minute)

Different tubes for different test…….some tubes have chemicals in it.
Safety Hazards in the lab
Exposed to four hazards blood born pathogens (blood in all and bodily fluids) 2. chemical hazards 3. electrical hazards 4. fire hazards
OSHA
The word occupational means pertaining to the workplace…..osha protects employees from occupational exposure of blood born pathogens or potentially harmful substances. Osha makes sure your boss makes sure your safe @ all times. Osha works with government to make sure you are working in a safe environment. The employer is responsible to follow all rules and reguations as well as enforce those regulations. CalOsha is more strict than OSHA.
OSHA
Meeting the OSHA standards is not _optional________________, failure will result in a fine that may total $_10,000_____________ for each employee

Further violations could shut _____down_____________the facility.
PPE
Gloves, jackets, masks, face shields, hair cover are all used to protect against blood born pathogens and fluids.

Gloves provide a barrier but can be penetrated by needles
The majority of needlesticks occur when health care workers
Dispose of their needles
*administer injections
*draw blood
*recap our needles
*handle trash and dirty linens
Do Safer Needle Devices Prevent Injury
Can’t eliminate all, but…
83% can be prevented
Needlesticks
800,000________ Needlestick Injuries Occur Each Year in the United States
Needlesticks
16,000_________of These Are Likely to Be Contaminated by HIV
Fact
HBV and HCV Pose an Even Greater Risk Then HIV
Clinical Laboratory improvement_Amendments
Designed to set safety policies and procedures that protect the patients.

_Regulated_the laboratory tests performed on specimens taken from the human body
Clinical Laboratory improvement_Amendments
Set up standards for all laboratory testing to ensure
_accuracy
_reliability_
_Timeliness
of patient test results regardless of where the test was performed

Regulations are based on the complexity of the tests performed and affect all aspects of the laboratory
CLIA
3 key factors considered in the regulations.
_type of test performed
Testing must be ordered by a physician
Certain laboratories can only performs tests according to their license.
Personnel involved in the testing
Certain tests must be performed by a licensed professional.
CMA’s have a limited scope of tests that they can perform
Quality control_
All equipment must be in good repair and working according to manufacture guidelines
Muscular System
Muscles provide heat and movement.
Muscles provide heat to our bodies
Skeletal System
Internal support
Framework
Produce blood
cells
Intergumentary system
Intergumentary is skin, hair, and nails and provides external support. It protects your body from pathogens and microorganisms
Lymphatic System
Body immunity produces Lymphocytes
Absorb fats
Drainage removes waste
Defense system
Endocrine System
Secrete
hormones
Respiratory System
2 bronchials, 1 windpipe…..gas is oxygen and carbon dioxide
Urinary System
2Kidneys, 2utiters, 1 bladderand 1 urethia
Filter blood
Remove waste
Nervous system
Control all
other body
systems
Digestive System
Absorb food
materials
Starts from the mouth includes the teeth, pharynx, esophagus, stomach, large intestines and small intestines…and the colon. Absorb nutrients from food we consume.
Male Reproductive System
Produce male
sex cells
(sperm)
Female reproductive system
Produce female
sex cells (ova)
Circulatory System
Vitamins,gasses,fats, and all goes thru circ. Sys from head to toe. Blue are veins and reps. Oxygenated bllod. The arteries are red and it usues blood that has n=been circulated through the body but needs oxygen again.
Transport
nutrients to
body
Blood Glucose testing
Aka blood sugar test or a fasting blood sugar test (fbs) also blood glucose test all mean you test for sugars but fasting blood sugar test is scheduled and it has to be the patient fasted for 8 to 12 hours and no black coffee. All test can be done by prick of finger or drawing blood from a vein so the specimen can be collected from the two diff. routes….GT glucose tolerance and GTT glucose tolerance test first they are given a nasty condensed soda and they come in fake colas give to the patient orally and the test occurs an hour after they want to make sure the pancreas dissolves the sugar and that’s how they test to see if a patient is diabetic.
Why get for tested for bgt?
To determine whether or not your blood glucose level is within normal ranges
To screen for, diagnose, and monitor Diabetes
Pre-diabetes
Hypoglycemia (low blood glucose)
When to get tested for bgt?
As part of a yearly physical
When you have symptoms suggesting hyperglycemia (high blood glucose) or hypoglycemia (low blood glucose)
If you are pregnant (gestational diabetes when getting diabetes when your preggers only)
If you are diabetic, up to several times a day to monitor glucose levels
Brain and Nervous System Functions
Brain and the nervous system cells not only rely on glucose for energy, they can only function when glucose levels in the blood remain within a narrow range.
Glucose hinges on the availability of insulin, a hormone produced by the pancreas.
Insulin acts as a traffic director, transporting glucose into the body's cells
Directing the liver to store excess glucose as glycogen.
We cannot live without glucose or insulin, and they must be in balance.
Normally blood glucose levels rise slightly after a meal
And insulin is secreted to lower blood glucose levels.
Hyperglycemia and Hypoglycemia
Hyperglycemia and Hypoglycemia, caused by a variety of conditions, are both hard on the body.
Severe, acute high, or low blood glucose levels can be life threatening causing:
Organ failure
Brain damage
Coma
In extreme cases, death
During pregnancy
What is it called? Gestational diabetes. Does it go away?

Untreated hyperglycemia that arises during pregnancy, in the form of gestational diabetes, can cause mothers to give birth to large babies who may have low glucose levels.
This condition can lead to brain and nerve damage
GLUCOSE TESTING
Be sure the patient has not eaten one to two hours before testing a regular finger test.
The sugars will be high if they don’t follow directions.
AREAS USED for glucose
Second and third finger
Clean the finger with the alcohol swab and prick with the lancet.

Apply a small drop of blood to the test strip on the monitor screen.
You have 2 minutes from this point to apply a blood sample.
Milk the finger until a large drop of blood is formed.
Wipe the first drop of blood from the finger with a cotton swab.


Milk the finger until a large drop of blood is formed.
Normal Ranges Fasting Blood
This means that the pt has not ate form 8 to 12 hours

Below 70 is hypo
Above 126 is hyper
POCT
Point of Care testing, done in the office.
Fast, easy and efficient.
Get results in minutes.
Easy way for physician to know if glucose levels are out of normal ranges.
Heel sticks for infants, ear lobes for infant, children and adults, but more common for children, adolescents, and adults is the finger stick.
phenylketonuria (PKU)
Inherited disorder of body chemistry, pku must be done within 24 hours of birth….if we let it pass and baby has pku and we pass 24 hours the baby can become mentally retarded….1 and 10,000 baby is born with pku.
PKU-Neonates Heel Stick
A phenylketonuria (PKU) test is done to check whether a newborn baby has the enzyme needed to use phenylalanine in his or her body. Phenylalanine is an amino acid that is needed for normal growth and development. If a baby's body does not have the enzyme that changes phenylalanine into another amino acid called tyrosine, the phenylalanine level builds up in the baby's blood and can cause brain damage, seizures, and mental retardation.
The damage caused by PKU can begin weeks after the baby has started drinking breast milk or formula. Babies with PKU need foods low in phenylalanine to prevent severe brain damage. Phenylalanine is found in most foods that have protein, such as milk, cheese, and meats.
It is important to find this disease early. All babies in the United States are tested for PKU right after birth. Each year, about 250 U.S. children get PKU.1 PKU occurs more in whites and Native Americans and is less common in blacks, Hispanics, and Asians. To have the disease, you must inherit the gene from each parent. The United States Preventive Services Task Force recommends that all newborns be tested for PKU.2
The blood sample for PKU is usually taken from your baby's heel (called a heel stick). The test is done in the first few days after birth, as early as 24 hours after birth. A follow-up test is usually done at age 7 to 10 days. A urine PKU test is done on a baby who did not have a blood test and who is older than 6 weeks.
Why It Is Done
A phenylketonuria (PKU) test is done to see whether a newborn baby has the enzyme to use phenylalanine in his or her body.
It is important to test your baby for PKU soon after birth to prevent brain damage. Phenylalanine blood levels in a baby with PKU start to rise within 24 hours of drinking breast milk or formula. A PKU test done 2 to 3 days after birth will show whether your baby has PKU. State laws say that newborn PKU tests must be done within a few days after birth.
How To Prepare
Your baby should be drinking breast milk or formula for 24 hours before the blood sample is taken. PKU test results are more likely to be correct if the blood sample is taken after the baby has been drinking milk or formula for at least 48 hours.
If your baby is older than 6 weeks, he or she will have a PKU urine test. You do not need to do anything before your baby has this test.
How It Is Done
Neonatal Capillary
v
Blood Collection Systems
When performing a venipuncture procedure all ways remember
+getting patient consent before the procedure
+explaining the procedure you will be performing to the patient
+keeping the patient information confidential
+appropriate PPE’s such as gloves and a lab coat
Vacuum (Evacuated Tube) Systems
Requires three components:
Evacuated sample tube
Double pointed needle
Special plastic holder called an Adapter
The most efficient, safest, and easiest method of collecting venous blood sample
Prior to the development of this system, patients who had more than one test ordered had to have multiple needlesticks!
Vacuum Needles
Double pointed needle
Multi-sample needle is 1-1 ½ inches long
Gauge is 21-23
One end of the needle pierces the patient’s vein
The other end, pierces a vacuum tube
Parts of A Needle 3 PARTS
A needle has three parts, the hub, the shaft, and the bevel. The hub is at one end of the needle and is the part that attaches to the syringe. The shaft is the long slender stem of the needle that is beveled at one end to form a point. The hollow bore of the needle shaft is known as the lumen. Disposable needles should always be used when preparing admixtures as they are presterilized and individually wrapped to maintain sterility.
Vacuum (Evacuated Tube) Systems
Evacuated Tubes
Can be used in both vacuum tube system and in the syringe method
Blood can be collected directly into the tube during the actual venipuncture

Tubes hold different amounts
The Syringe System
Used less often than the vacuum tube method
This system is preferred for veins that can collapse under a vacuum:
Small children
Geriatric patients
Fragile surface veins
Small veins
Veins that roll
Veins on the back of the hand or from the foot
The Syringe System
Hypodermic needle
Disposable plastic syringe
Winged Infusion System
Combines both the benefits of the syringe system and the vacuum tube system
Used for small veins that are difficult to puncture and have a tendency to collapse
Winged Infusion System
21 to 23 Gauge with attached plastic wings
23 G is the most common size
6 or 12 inches of tubing leads from the needle
On the other end of the tubing is a hub that can attach to a syringe
Winged Infusion System
The butterfly needle slides into a small surface vein in the back of the hand, wrist, or foot
The needle is inserted at a 5 to 10 degree angle and then threaded into the vein
winged infusion
1 butterfly wing infusion
2 adapter needle holder
6 vaccum tube
3 double ended needle
4 barrell
5 hub
Packaging identifies the gauge and the length
Greet and Identify patient Ask about allergies
Greet and identify pt….really ask about latex allergies

Most important steps to blood draws
Name
DOB
Consent/verbal is fine
Medical Record Number
Identify the patient
Veins Used for Blood Collection
Basilic, Cephalic, Median Cubital
Best Available sites in order
Best Available sites in order:
Antecubital
Hand
Wrist
Foot
Perform the venipuncture
Less pain will be experienced if the bevel of the needle is up
The needle should be inserted at a 10 to15(what she said in class) to 30 degree angle to the surface of the skin
Never go lower than 10 but u can do 25
Cholesterol is an essential fat for our bodies
Our body makes up 80 percent of choleserol with the help of the liver.
We use the cholesterol to make hormones

Cholesterol is a waxy substance that our bodies need to maintain the function of the nervous system.this includes the function of the skin, muscles, liver, intestines and the heart.

Dietary cholesterol is the kind we eat…dietary means pertaining to what u eat…it comes only from animal origin…
Cholesterol
HDL : “Good” Cholesterol

LDL: “Bad” Cholesterol

Total Cholesterol
cholesterol
Hdl chol. Protects arties from forming plaque.

HDL- high density lipoprotein “good” cholesterol


A low hdl cause stroke and coronary artery disease…..

Ldl- bad cholesterol
Ldl- causes plaque fomation and makes blood sticky and also you clot faster.
Too much ldl can affect the brain, heart and lungs.
This condition is called atherosclerosis (plaque build up) this can leave to stroke or cad(coranary artery disease)


Blood thinners will open up a clot….
cholesterol
Cholesterol levels provide knowledge about your potential for cardiovascular disease (cardiovascular means pertaing to the vessells of the heart)

Angina (means pain)
TIAS
Arterial disease is all attacks of the heart but is not quite a heart attack.
Total Cholesterol LDL & HDL = Total Cholesterol
Total cholesterol
Desirable level: 180 to 200 mg/dl
Borderline risk: 200-240 mg/dl
Undesirable risk: above 240 mg/dl
LDL
Low-density lipoprotein (LDL) or "bad" cholesterol Desirable level:90-130 mg/dl
Borderline risk:130-160 mg/dl
Undesirable risk:anything above160 mg/dl
HDL
High-density lipoprotein (HDL) or "good" cholesterol Desirable level: above 45-65 mg/dl
Undesirable risk:below 35 mg/dl
TRIGLYCERIDE
Energy
Excessive amounts= adipose tissue.
Desirable:less than 150
Skin Punctures
Dermal punctures
Capillary punctures
Cafinger sticks
Capillary Sampling
Using a rolling movement of your thumb, lightly press and “Milk” the finger from the top knuckle towards the tip.
This stimulates the flow of blood towards the sampling area.
Hematocrit Testing
Alternative name: HCT
Referred to as packed RBC volume
A hematocrit test is part of the Complete Blood Test
Can be individually ordered by the physician rather than an entire CBC
Formed Elements Red blood cells
RBCs
Erythrocytes 
Function: transport oxygen and carbon dioxide to deliver iron
Formed Elements White blood cells
WBCs (leukocytes)
 Function: general and specific immunity to pathogens invading the body
Formed Elements Platelets
Platelets are cells that contain many chemicals including ones that help seal broken blood vessels
Anemia
A low hemoglobin measurement usually means the person has anemia.
Anemia results from a decrease in the number, size, or function of RBCs.
Common causes include excessive bleeding, a deficiency of iron, vitamin B12, or folic acid or mechanical trauma
What does the test result mean?
Normal values in an adult are 12 to 18 grams per deciliter (100 milliliters) of blood.
Men 14.0-18.0
Women 11.5-16.0
Sickle cell anemia is an inherited blood disorder, characterized primarily by chronic anemia and periodic episodes of pain
A low hemoglobin measurement usually means the person has anemia.
Anemia results from a decrease in the number, size, or function of RBCs.
Iron Deficiency Anemia
Most frequently seen in an ambulatory care setting
There are many different types and locations of medical laboratories
size
capability
affiliations
Independent Laboratories
Located within a medical center or large clinic
Receive samples from:

Specimens are collected there and are taken back to lab for centeral lab processing.
Reference Laboratories
These are known to be independent labs there used by different hospitals or by physicians for more complex or specialized test…more expensive….specific DNA test….complex comprehensive test
Point-Of-Care-Testing POCT
Also known as near patient testing or bedside test…the point of this is to generate a result quickly so that appropriate treatment can be implemented. We Do POCT in clinics alot
Type Of POCT Tests
Electrolyte systems
blood gas
hand held single use
Blood gas is they draw ur blood from iv port and its measuring how much oxygen you have and then it goes back in your body. handh
Physician Office Laboratory
Common medical test can be performed and inexpensively performed in the doctors office by the MA
Laboratory Departments
Test whats inside whole blood…red blood cells, white blood cells, esr testing,,,,they asses components of whole blood in platelettes…
Parasitology Department
Where ova and parasite testing or testing…..means little bugs that are inside blood or body….the parasites come out in stool and there looking for scabies, penworms and maleria. We test in clinic for this