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31 Cards in this Set
- Front
- Back
why do we want to know what microbe is causing a disease
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wrong antibiotic could be prescribed
side effects virus does not respond |
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what technique is used to collect a speciman
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aseptic technique
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the three different mechanisms used to id a microbe
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phenotypic genotypic immunilogical
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using phenotypic methods what are the three different methods to I.D. a microbe
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gram staining,selective and differential media,biochemical tests
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what are some examples of selective and or differential media
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blood agar,mannitol salt agar,look for AGAR
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what are some biochemical tests
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look for enzymes such as ase- ,tubes, API coagulase-
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pcr
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polymerase chain reaction
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specific targets of pcr
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dna sequence rRna genes,toxin genes
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what things do you need to run a pcr reaction
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The The d's dna sample,dna polymerase,deoxynucleotides,ATP primers,thermocycler
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helical viruses name all of them
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r-rabies, I- influenza, M-measles E-Ebola
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signs
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objective and measurable
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sequalae
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after - effect ex: strep throat can turn into scarlet fever
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viral diseases tend to have-------
incubation periods than bacteria |
longer
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the one exception of the above is
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leprosy
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portals of exit
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mostly areas that you want to avoid-resp,salivary,skin,fecal,urogenital,blood.
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drawbacks of phenotypic methods
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variable,isolation needed,time,fail,labor,not all grow on media
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genotypic methods are based on
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DNA genes,sequences
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main concern with genotypic methods
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detecting a signal--- amplification.
DNA is amplified in genotypic methods. |
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PCR is used to amplify dna specifically
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rRna, toxin genes,virulence factors
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ELISA
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enzyme linked immunosorbent assay
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drawbacks of immunological assays
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finding good antibody,maintaining good antibody,cross reactivity,temp sensitive
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etiology
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causative agent
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how do you view a virus what microscope is used
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electron microscope
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viruses infect
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animals,protists,fungi.bacteria,plants.... all.
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polyhedral viruses include
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polio,adenovirus,hep b
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complex viruses include
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look for pox in this word -chicken pox,cow pox,small pox
LPS |
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viruses sometimes have
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spikes and envelopes
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enveloped viruses are destroyed by alchol true or false
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true
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enveloped viruses are easy to destroy
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true
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the viral multiplication cycle depends on the type of virus and the host
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b-bacterial viruses
a-animal viruses retroviruses |
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adsorption,penetration,uncoating,syntheis,assembly,release, multiplication in
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animals
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