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31 Cards in this Set

  • Front
  • Back
why do we want to know what microbe is causing a disease
wrong antibiotic could be prescribed
side effects
virus does not respond
what technique is used to collect a speciman
aseptic technique
the three different mechanisms used to id a microbe
phenotypic genotypic immunilogical
using phenotypic methods what are the three different methods to I.D. a microbe
gram staining,selective and differential media,biochemical tests
what are some examples of selective and or differential media
blood agar,mannitol salt agar,look for AGAR
what are some biochemical tests
look for enzymes such as ase- ,tubes, API coagulase-
pcr
polymerase chain reaction
specific targets of pcr
dna sequence rRna genes,toxin genes
what things do you need to run a pcr reaction
The The d's dna sample,dna polymerase,deoxynucleotides,ATP primers,thermocycler
helical viruses name all of them
r-rabies, I- influenza, M-measles E-Ebola
signs
objective and measurable
sequalae
after - effect ex: strep throat can turn into scarlet fever
viral diseases tend to have-------
incubation periods than bacteria
longer
the one exception of the above is
leprosy
portals of exit
mostly areas that you want to avoid-resp,salivary,skin,fecal,urogenital,blood.
drawbacks of phenotypic methods
variable,isolation needed,time,fail,labor,not all grow on media
genotypic methods are based on
DNA genes,sequences
main concern with genotypic methods
detecting a signal--- amplification.
DNA is amplified in genotypic methods.
PCR is used to amplify dna specifically
rRna, toxin genes,virulence factors
ELISA
enzyme linked immunosorbent assay
drawbacks of immunological assays
finding good antibody,maintaining good antibody,cross reactivity,temp sensitive
etiology
causative agent
how do you view a virus what microscope is used
electron microscope
viruses infect
animals,protists,fungi.bacteria,plants.... all.
polyhedral viruses include
polio,adenovirus,hep b
complex viruses include
look for pox in this word -chicken pox,cow pox,small pox
LPS
viruses sometimes have
spikes and envelopes
enveloped viruses are destroyed by alchol true or false
true
enveloped viruses are easy to destroy
true
the viral multiplication cycle depends on the type of virus and the host
b-bacterial viruses
a-animal viruses
retroviruses
adsorption,penetration,uncoating,syntheis,assembly,release, multiplication in
animals