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36 Cards in this Set
- Front
- Back
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What are some diseases caused by spore forming bacteria?
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tetnas
botulism |
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What staining techniques are differential?
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Gram staining
Acid fast staining Spore staining |
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What stains do you NOT heat fix the smears?
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Negative staining
Capsule staining |
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What color result will occur with Gram +?
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Blue/Purple
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What color result will occur with Gram - ?
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Red/Pink
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What is the color result for a non-acid fast?
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Blue
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Some bacteria have mycolic acid in the cell walls. What is a good stain for these bacteria
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Acid fast
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What is the color result for an acid fast bacteria
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Red/pink
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What dyes are used for Acid fast staining
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Carbolfuchsin & methylene blue
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Acid fast staining is an important diagnostic tool in the identification of?
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Mycobacterium tuberculosis
Mycobacterium lepre |
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M. smegmatis is a good control in acid fast staining. What is the result of M. smegmatis?
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Acid fast
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S. aureus is also a good control in acid fast staining. What is the result of S. aureus?
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non-acid fast
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What is a stain good for Clostridium?
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spore stain
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What stain is good for Bacillus?
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spore stain
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What bacteria are capsule stain for?
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Klebsiela pneumonial
Streptoccus pneumonial |
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What type of stain is this?
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Spore staining
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What is the purpose of Simple stain?
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Quick, morphology, arrangement, sometime inclusions
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What is the purpose of Negative stain?
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Very fast, morphology, arrangement, ACCURATE SIZE
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What is the purpose of Capsule stain?
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For viewing capsule
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What is the purpose of Gram stain?
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simple staining test that simply identifies the two main groups of bacteria. Gram positive, and gram negative.
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What determines why something would test Gram +?
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Thick peptidoglycan layer
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What is the purpose of Spore staining?
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Makes endospores visable
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What is the purpose of Acid fast staining?
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To identify Acid-Fast bacteria (AFB) organisms covered with a hard waxy outer layer called lipoid capsule
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What are the most common acid fast bacteria?
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Mycobacterium tuberculosis &
Mycobacterium leprae |
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What is the primary stain of spore staining?
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malachite green
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What is the secondary stain of spore staining?
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safranin
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What type of stain technique is used in Capsule staining?
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combo
negative & simple |
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What stains are used for capsule staining?
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carbolfuchsin & congo red
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What happens with the pathogenic and phagocytosis with having a capsule
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increased pathogenic and decreased phagocytosis
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What is the counter stain in Acid fast staining?
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Methylene Blue
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What is the primary stain in Acid fast staining?
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Carbolfusin
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What is the counter stain for spore stain?
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safranin
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What is the primary stain for spore staining?
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Malachite green
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What is the primary stain for Gram staining?
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Crystal Violet
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What is the mordant in gram staining?
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Iodine
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What is the counter stain in gram stain?
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safranin
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