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36 Cards in this Set
- Front
- Back
- 3rd side (hint)
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• coccobacilli • obligate aerobe • NM (except B. bronchiseptica) • oxidase (+) (except B. parapertussis) • urease (+) (except B. pertussis) |
What are the general characteristics of Bordetella species? |
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Bordetella species |
Culture appear as smooth, glistening, silver in color becoming whitish gray with age. |
SSW |
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Growth factors: • Nicotinic acid • Cysteine • Methionine |
Growth factors of Bordetella species |
NCM |
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Virulence factors: • Pertussis toxin • Adenylate toxin • Cyclase • Tracheal cytotoxin • Dermonecrotic toxin • Pertactin • Fimbriae |
Virulence factors of Bordetella species |
PACTDPF |
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Bordetella pertussis |
Bordet Gengou Bacillus |
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• obligate aerobe • gram (-) minute coccobacilli • NM except B. bronchiseptica • NS • hemolytic organisms |
What are the morphological characteristics of Bordetella pertussis? |
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• Stuart's medium • Mishullow's charcoal agar |
Transport media for Bordetella pertussis? |
SM |
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Bordet Gengou media |
Contains Potato Blood Glycerol |
Cultivation for Bordetella pertussis |
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Jones Kendrick Charcoal Agar |
Contains charcoal and yeast extract |
Cultivation for Bordetella pertussis |
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Regan-Lowe Medium |
Contains charcoal and horse blood |
Cultivation for Bordetella pertussis |
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Charcoal Cephalexin Blood Agar |
Preferred because it contains horse blood, charcoal, cephalexin and amphotericin |
Cultivation for Bordetella pertussis |
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Casamino broth/ Stainer and Scholte |
Incubated at 35C without elevated CO2 for 12days for Bordetella pertussis |
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Whooping cough |
Highly contagious, acute infection of URT for children |
Airborne transmission |
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• Catarrhal stage • Paroxysmal stage • Convalescent stage |
3 stages of the disease |
CPC |
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Catarrhal stage |
Runny nose; mucuos membrane inflammation |
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Paroxymal stage |
Continuous coughing that ends in respiratory whoop; severe and violent coughing associated with vomiting and whooping; may last for 6 weeks |
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Convalescent stage |
Can last for as long as 6 months after infection |
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Bordetella pertussis |
Requires 7-14 days of incubation |
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• Pertussis toxin • Hemagglutinins (F-HA and PT-HA) • Adenylate cyclase • Heat labile toxin • LPS, heat stable toxin • Tracheal cytotoxin |
Pathogenecity: Toxins and Enzymes of Bordetella pertussis |
PHAHLT |
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Nasopharyngeal aspirates and swabs |
Ideal specimen for laboratory diagnosis of Bordetella pertussis |
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• Stained smears • Culture • FAT • Slide Agglutination Test |
Laboratory diagnosis for Bordetella pertussis |
SCFS |
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Bordetella parapertussis |
• NM • urease and oxidase (+) • nitrate (-) • citrate (+) |
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Bordetella parapertussis |
Large colonies with brown pigment on Bordet Gengou agar |
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Bordetella parapertussis |
Causes milder form of pertussis-like symptoms |
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Bordetella bronchiseptica |
• Motile • urease and oxidase (-) • nitrate and citrate (+) • causes septicemia • inhabits respiratory tract of canines |
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Bordetella bronchiseptica |
Kennel's cough |
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Urease:
(-) Bordetella pertussis (+) Bordetella paraper (+) Bordetella bronchi |
Identify the Urease test for: ? Bordetella pertussis ? Bordetella paraper ? Bordetella bronchi |
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Motility: (-) Bordetella pertussis (-) Bordetella paraper (+) Bordetella bronchi |
Identify the Motility test for: ? Bordetella pertussis ? Bordetella paraper ? Bordetella bronchi |
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NO3: (-) Bordetella pertussis (-) Bordetella paraper (+) Bordetella bronchi |
Identify the NO3 test for: ? Bordetella pertussis ? Bordetella paraper ? Bordetella bronchi |
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Oxidase: (+) Bordetella pertussis (-) Bordetella paraper (+) Bordetella bronchi |
Identify the Oxidase test for: ? Bordetella pertussis ? Bordetella paraper ? Bordetella bronchi |
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Franciscella tularensis |
Classified as Bacterium tularense |
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Francisella tularensis |
Classified as Brucellan tularense |
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Franciscella tularensis |
Classified as Pasteurella tularensis |
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Franciscella tularensis |
Jellison type A Tularemia |
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Jellison type A Tularemia |
Affects rodent, rabbits, squirrels and chipmunks |
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• being bitten by an infected tick, deerfly or other insect • handling infected animal carcasses • breathing in bacteria, F. tularensis • Tularemia is not known to be spread from person to person |
How does tularemia spread? |
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