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8 Cards in this Set
- Front
- Back
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Definitions
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Catalyst: Substance that increases the rate of RXN
Active Site: Pocket that contains AA side chain that creates a structure that binds substrate. Holoenzyme: Enzyme cofactor complex. Enzyme without factor:apoenzyme Cofactors: Additional molecule Coenzymes: small organic molecule that serves as a cofactor. Catalytic efficiency: Number of molecules product produced/second. Compartmentalization: Enzymes localized to specific compartment. |
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How do Enzymes work?
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V=kA, k is rate constant.
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Ea
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Energy Activation: Energy required to achieve the activated enzyme:substrate transition state. Catalyst lowers Ea.
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Induced fit is the correct model.
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Not lock and key bitch.
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What to do with M-M equation:
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V0= Vmax[S]/[S+Km]
v- is initial. Vmax=maximum. Km= [S] at which V=Vmax/2. Lower Km= higher RXN velocity. |
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Lineweaver Burk
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1/S vs 1/V Straight Line.
Xint: -1/Km Yint; 1/Vmax Initial reaction rate is measured at a variety of substrate concentrations. Here, Km can be determined. |
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Competitive Inhibitors
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No change in Vmax, higher Km doe.
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Reversible enzyme inhibition
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Noncompetitive: Vmax is lowered, Km does not change. Binds other than substrate binding.
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