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5 Cards in this Set
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Blood: % plasma, cells?
Name for oxidation products of methylene blue, used in staining: Three azure dyes used in staining RBC's: Colors of basophilic, eosinophilic, neutrophils: |
55% plasma, 45% cells
azures Giemsa, Wright's, Leishman's baso - blue-purple-black eos - red, pink neuts - reddish brown, neutral color |
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Three stages of embryonic hematopoiesis, and structures involved:
Differences between infant and child marrow: Structures in red marrow: |
Primordial - yolk sac --> AGM - primitive erythroblasts; requires BMP
Hepatosplenothymic - Weeks 4-8; highest hematopoietic activity Medullolymphatic - Weeks 11-12; completely transferred to bone marrow HSC's restricted to marrow; liver/spleen can help make RBC's Infant - red, child - yellow (more fat) stroma - reticular CT, adipocytes, m0's, hemonectin (binds cells to stroma) cords - packed/overlapping maturing blood cells + vascular sinusoids |
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Explain the monophyletic theory of hematopoiesis:
Erythropoiesis: 6 stages, explain how they are arranged: |
All BC's arise from a common stem cell (pluripotential --> multipotential --> progenitor cells -> precursors, -blasts)
PBPORE - arranged by cytoplasmic staining, nuclear size/chromatin, cell size Pro-E-blast - 14-19 um - big nucleus, +mitosis Basophilic E-blast - 13-16 um - patchy chromatin, + mitosis Polychromatic E-blast - condensed, gray cytoplasm Orthochromatophilic E-blast - small nucleus, acidophilic, NO mitosis Reticulocyte - immature RBC, Cresyl Blue dye, ~1% circ. Erythrocyte |
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6 Stages of granulopoiesis:
Which stage has the biggest cells? Higher number of what stage usually indicates bacterial infection? |
My Parents Made Me a Marching Band Geek:
M-blast - large, spheric nucleus, +mitosis, no granules Pro-M-cyte - flattened nucleus on one side, azurophilic, no specific granules, +mitosis M-cyte - indented nucleus, SPECIFIC granules (neuts, eos, basos); + mitosis Meta-M-cyte - deep, indented nucleus, more packed chromatin, granules Bands - horseshoe-shaped nucleus, more condensation, lobulation --> mature granulocytes Pro-M-cyte - 15-24 um Bands |
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How are monocytopoiesis and lymphopoiesis alike?
Explain thrombopoiesis: |
Both have a -blast stage, then pro_____ stage.
Megakaryoblast - 15-50 um - ovoid, kidney-shaped large nucleus, basophilic Megakaryocyte - 100 um - endomitosis, increased DNA replication, membrane demarcation Thrombocytes (platelets) |
