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12 Cards in this Set

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Define Sterile
environment free of microbes, viruses, & endospores


***ONLY thing 100% free of microbes
Define Disinfection
process which eliminates most disease-causing microorganisms & viruses from a surface
Define Pasteurization
heat tx to reduce # of microorganisms capable of spoiling food

(moderately heat moist)
Define Decontaminated
item that has been treated to reduce # of disease-causing microorgaisms to a "safe" level
Selection of antimicrobial procedure is based on what (4) factors?
type of microorganisms
number of microorganisms
environmental conditions
potential risks of infections
Diff btwn critical, semi-critical, & non-critical items
critical- directly contact & penetrate body tissues (needles, etc)
semi-critical- directly contact mucous membranes (endotracheal tubes, etc)
non-critical- directly contact unbroken skin (stethoscope, etc)
__________ involves pressurized steam. Items are sterilized at 15 psi, 121 degrees C, for 15 minutes.
Autoclaving
Bacillus stearothermophilus requires high heat to kill. What is its optimal growth temperature?
55 C


(heat sensitive indicator tape can be used to check sterilization)
Germicidal chemicals, such as ______________ can be used to sterilize DNA
ethylene oxide
How does ultraviolet radiation sterilize?
damages DNA creating thymine dimers
creates kinks in DNA helix making replication difficult

(doesn't kill but makes replication impossible)
Presence of what items can interfere w/ sterilization?
dirt, grease, & bodily fluids
(clean first, then sterilize)
Difference btwn bactericidal & bacteriostatic
bactericidal- KILLS bacteria

bacteriostatic- prevents bacterial growth (doesn't kill)