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15 Cards in this Set

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What is a hemoglobin?

Protein in RBC which carries O2 to tissues and CO2 to the lungs

____________ in ____ which __________ to _________ and _______ to the _________.

Hemoglobin



Normal values


Male vs. Female

Male is higher than female



Notes: data

male adult: 13.5 to 17.5 g/dL


Female adult: 12 to 16 g/dL

Hemoglobin Measurement



Whole blood is mixed with Drabkins reagent



Mechanism?

Ferricyanide converts the iron from the ferrous (+3) to ferric state (+2) to form methemoglobin, which then combines with potassium cyanide to form the stable pigment cyanmethemoglobin.

Hemoglobin Measurement




Mechanism

Ferricyanide converts the iron from the ferrous (+3) to ferric state (+2) to form methemoglobin, which then combines with potassium cyanide to form the stable pigment cyanmethemoglobin.


This is read on a spectrometer at 540 nm.

____________ the _____ from the _______________ to _________ to form _____________, which then ____________ with ___________ to form the _____________________. This is __________ on a __________ at ____________.

Hematocrit



Def.

Percent of RBC volume in whole blood.

_________ of _____________ in _____________.

Hematocrit



Normal values



Male vs. female

Male is larger than female

Male adult: 41-53%


Female adult: 36-46%

How hematocrit measurement is prepared?

The whole blood is centrifuged.

The _________ is _____.

Hematocrit measurement

After the whole blood is centrifuged, measure the space occupied by the packed RBC - Hematocrit.

After the _________ is ________, _________ the _______________ by the ___________.

How hematocrit is expressed?

As percentage of RBC in a volume of whole blood.

As _________ of _______ in a _______ of _____________.

What hematocrit is used to evaluate?

Anemia (Decrease of red blood cells)



Polycythemia (Increase in RBC)



Dehydration



Decisions on blood transfusion



Effectiveness of blood transfusion

________ (Decrease of ________)


_________ (Increase in ______)



De_________________



Decisions on ___________


Effectiveness on ___________

What hematocrit is used to evaluate?



________________ (decrease of RBC)



_____________ (Increase in _______)



D_________________


Decisions on _______________


Effectiveness on ____________

Anemia


Polycythemia



Dehydration



Decisions


Effectiveness

What hematocrit is used to evalaute?

_________ (______ of ________)


__________ (________ in ________)


________________


____________ on ________


_____________ of __________

Anemia


decrease


RBC



polycythemia


increase


RBC



dehydration



Decisions


blood transfusion



Effectiveness


blood transfusion

Rule of Three

Hgb (g/dL) x 3 = Hct (%)

______ (g/dL) x 3 = ________ (%)

Rule of three



Hgb (_____) x __ = Hct (___)

g/dL


3


%

Rule of Three

________ (_____) x ____ = ___ (___)

Hgb


g/dL


3


Hct


%