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7 Cards in this Set
- Front
- Back
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What can a respirometer be used for? |
It can be used to measure the rate of aerobic respiration by measuring the volume of oxygen consumed over time |
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How do you use it? |
You set up the apparatus and place it in a water bath at an optimum temperature for enzyme activity in the organism. After it equilibriates, the tap is closed and syringe is added and used to reset the manometer so the fluid is at the same level at both sides. Initial volume is recorded |
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What happens after this has been set up? |
As the organism respires, oxygen is consumed and an equivalent amount of CO2 is produced and then absorbed by KOH. This reduces pressure, causing the fluid to move towards the organism. |
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What tells us the volume of oxygen consumed? |
The difference between the initial and final reading |
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What happens if the respirometer is set up with water instead of potassium hydroxide? |
The volume of oxygen consumed= volume of CO2 produced if RQ=1 |
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What happens if other respiratory substrates are respired? |
Less CO2 is produced than oxygen consumed per gram |
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How would you work out the volume of CO2 produced when KOH is used? |
Volume of O2 calculated- change in volume |
