Quarter 2, Biochemistry, Test 2, Applied Molecular Biology

Front 1

the same gene of two individuals may be cleaved differently by a given restriction enzyme resulting in gene fragments of different legnth which is known as...

Back 1

restriction fragment length polymorphism (RFLP)

Front 2

what technique can be used to rapidly determine genotype of patient by hybridization of allele specific oligonucleotides to PCR amplified region of gene in question

Back 2

allele specific oligonucleotides

Front 3

hypervariable DNA contains a variable number of ____ which can be used to differentiate individuals

Back 3

tandem repeats

Front 4

what process can allow an individual to determine what genes are being expressed in a cell

Back 4

gene chips or microarrays

Front 5

gene delivery can occur via viral or non-viral vectors but non-viral has low ___ and viral vectors tend to induce ____

Back 5

efficiency/immune response

Front 6

what viral vector infects cells with high efficiency and integrates genes into genome, but only infects dividng cells and mest be rendered safe prior to use

Back 6

retrovirus

Front 7

what viral vector can infect dividing or non dividing cells, but is only transient and may induce immune response

Back 7

adenoviral vector

Front 8

which viral vector integrates gene into genome at defined site, is non-pathogenic, infects non-dividing cells, but lacks a good packaging cell line and only incorporates small DNA fragments

Back 8

adeno-associated virus

Front 9

what disease results in brain degeneration and death by about 10 years of age

Back 9

Canavan's disease

Front 10

what is needed to make a safe retrovirus

Back 10

helper provirus

Front 11

the helper provirus is unable to reproduce itself, but can express what viral proteins

Back 11

GAP, POL, and ENV

Front 12

is gene therapy more successful if there is no gene present or some gene present

Back 12

some gene present

Front 13

it was shown that an induced ___ caused the formation of leukemia in two SCID patients

Back 13

retrovirus

Front 14

10 patients have undergone Adenosine Deaminase Defeiciency gene therapy via retroviral vectors and what has been the affect

Back 14

increased immune function

Front 15

patients who were possible candidates for amputation were given ___ to stimulate angiogenesis

Back 15

VEGF

Front 16

in an effort to treat familieal hypercholesterolemia hepatocytes were treated with ___ that contained LDL receptor

Back 16

neurovirus