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19 Cards in this Set
- Front
- Back
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define fluorescence?
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Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.
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Is the emission or absorption wavelength longer?
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emission
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How long does a fluorescence molecule hang out in the excited state?
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nanoseconds
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Fluorescence emission spectra are roughly the ____________ of their absorption spectra.
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mirror image
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Fluorescence emission is ______________ dependent.
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solvent
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Flourescence ___________ is solvent dependent.
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emission
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This is why the So transistions for the _________ spectrum and ___________ spectrum do not always overlap.
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absorption, emission (fluorescence)
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I=
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fluorescent intensity
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k'=
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instrumental parameters, must be calculated
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Po=
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incident light, molecule dependent
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ɸ=
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efficiency of flourescent quantum yield
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Eex=
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molar absorptivity at Po
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C=
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concentration
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Slope =
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k'(PoɸEex)
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