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10 Cards in this Set

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What does mass spectrmetry measure?
The ratio of mass to charge
What are the three basic steps of all mass spectrmetry?
1. Convert neutral atom and molecules to ions (often radical cations)
2. Seperate ions based on mass to charge ratio
3. Measure abundance of each type of ion
Why does ionization of a molecule with electron bombardment form a radical cation?
The high energy electron removes an electron from either a lone pair or a bonded pair thus increasing the charge to 1+ but leaving a lone electron
What is a gentle, effective method of mass spectrmetry for large or biological molecules?
Electrospray ionization mass spectrometry
What is the resolution of a mass spectra the measure of?
It's ability to seperate ions of different masses?
In mass spectrometry, what is the basepeak?
The peak assigned to the most abundant ion. It is arbitrarily given the value of 100 and all other abundances are expressed as a percentage of this value
Low-resolution mass spec can detect ions at what difference in masses?
One or more amu
High-resolution mass spec can detect ions at what difference in masses?
As little as .0001 amu
How do isotopes affect mass spec?
They can give rise to an M+2 or M+1 peak. A molecule with lowly occuring carbon-13would be one amu greater than the same molecule with carbon-12 and thus would be found at M+1
What three elements commonly give M+2 peaks?
Chlorine, bromine, oxygen