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14 Cards in this Set
- Front
- Back
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What is genomics?
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The study of the genome of an organism. Based on nucleic acids and involves base-pairing and sequencing
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What are some techniques for genomics?
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Southern Blot (DNA Target), northern blot (RNA target), PCR, microarrays
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What is proteomics?
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The study of the "proteome" of an organism. Includes modified proteins and has a more challenging methodology than genomics.
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What is it important to measure changes in RNA/protein levels?
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It is a method to measure regulation at the RNA or protein level.
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When is genomics NOT useful?
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It is not useful if the sample does not contain nucleic acids. Ex: Saliva, urine, CSF, plasma
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What are microarrays used for?
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It measures RNA only, not protein. It can measure changes in mRNA on a genome-wide scale.
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What is SDS-PAGE used for?
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For analyzing proteins. When bound to SDS, proteins becomes negatively charged.
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What is a Western blot?
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Used in conjunction with SDS-PAGE. Uses 2 antibodies to detect specific proteins.
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What is Isoelectric focusing (IEF)?
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Used to resolve individual proteins. A pH gradient using ampholytes is established and proteins migrate to their isoelectric point. Then SDS-PAGE is performed.
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What does Trypsin cleave?
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The carboxyl side of lysine (K) and arginine )R) residues
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What are some advantages of using MS in screening?
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Very fast, sensitive, discriminating and allows for simultaneous testing.
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What is Phenylketonuria (PKU)?
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It is a genetic disease in which the conversion of Phe to Tyr is absent or greatly decreased. Phe levels are high and causes problems. MS/MS tests for it
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What is an indication that a child has PKU?
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On the MS the Tyr peaks are very low
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What is a norovirus and how can it be detected?
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It is a highly contagious RNA virus - gastroenteritis. It can be rapidly detected by MS (the capsid protein is detected in human stool)
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