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23 Cards in this Set
- Front
- Back
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What happens in the elogation cycle of protein synthesis?
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addition of amino acids from the tRNA in the 'P' site to the amino acid on the tRNA in the 'A' site.
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What do many anitbiotics do?
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They block stages in protein synthesis.
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What does tetracycline do?
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It blocks the 'A' site.
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What does chloramphenicol do?
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It interfers with translocation
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Peptidyl transferase activity is associtated with what
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The 23s rRNA
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Elogation is catalyzed by what?
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Peptidyl transferase activity.
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Amino acids move from the tRNA in the __ site to the tRNA in the __ site
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'P' , 'A'
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When the amino acids move over one codon this is called ?
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translocation
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After translocation the empty tRNA in in the __ site. The heavily forming polypeptide(or protein) is in the __ site and the __ site is empty.
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'E', 'P' and 'A'
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The naked tRNA from the 'E' site is ?
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recycles and reused.
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How is protein synthesis stopped?
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When the ribosome encounters a stop codon on the mRNA. (UAA, UAG, UGA)
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What happens after protein sythesis?
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Hydroloysis of the newly formed polypeptide occurs from the tRNA and the protein is released.
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For hydrolysis to occur we see ?
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three release factors.
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In proks we see simultaneous ___ and ___ and we also can see "_____ _____"
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transcription, translation, and cotranslational secretion.
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Who won the nobel prize for describing protein secretion in Euks?
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Gunther Blobel
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What do the chaperons do?
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They recognize info at aminoterminus of the new polypeptide and bind to it and aid in secretion through the cytoplasmic membrane.
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What does secB do?
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Interacts with the nascent polypeptide and reduces or retards the folding rate if the protein keeping it export competent
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What does secB do with secA?
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They bring the polypeptide to the secYEG translocase in the membrane.
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Protein exported via the General Secretory Pathway posses a __
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signal sequence at the Amino terminus.
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The GSP protein contains
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1)Charged amino acids(5-10)
2)Hydrophobic amino acids(15-20) 3)Acidic and posses a cleavage site(6) |
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The signal peptide is ____
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left behind(cleaved from the protein)
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Gene expression is __ __.
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Highly regulated
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What does highly regulated mean?
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All the genes are not always turned on. SO genes to 'deal' with a particular environmental condition are selectively expressed.
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