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10 Cards in this Set
- Front
- Back
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Order of microscope powers
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A. Low power
B. High-dry C. Oil immersion |
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What are the two things you do at low power in a diff?
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1. Determine nonrandom cell distribution - a. fringe analysis
b. rouleaux/agglutination c. platelet clumping d. look for artifact 2. Select the best area for a diff |
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What occurs at the High-dry power in a diff?
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WBC estimate.
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How do you do a WBC estimate?
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1. Area: RBC overlapping slightly
2. Average #WBCs in 5 fields. 3. Multiply x 2000 = WBC estimate 4. See if estimate agrees with real count. |
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What do you do after a WBC estimate?
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switch to oil immersion for the 100-cell diff. but FIRST DO A STAIN AND SMEAR EVAL
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what do you look for when evaluating
-stain -smear |
Stain: excessively blue, excessively pink, or precipitated stain.
smear: Water contamination, echinocytes everywhere, thickness/length of the smear. |
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What cells are not included in the 100 WBC count/id?
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nRBC
Smudge cells Giant platelet forms |
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what do you do if you find a nRBC?
how? |
correct the WBC:
100 x WBC --------- = Corrected WBC 100 + nRBC |
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what do you do when done with the 100 ID/count?
How? |
platelet estimate.
count and avg # platelets in 6 fields, multiply by 15 |
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when should you repeat a diff?
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when abnormal cells are seen
nRBC's are seen immature cells WBC < 2000/ul |
