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28 Cards in this Set
- Front
- Back
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What are the 5 I's?
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Inoculation
Incubation Inspection Isolation Identification |
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Define: Inoculation
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placed in a container containing sterile culture media
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Define: Incubation
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placed sample into an incubator. right temperature, gas content that promotes the growth of microbes of interest
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Define: Inspection
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Observation
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Define: Isolation
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Further culturing to obtain pure culture
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Define: Identification
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Morphology, staining, biochemical tests, molecular tests
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What is semi solid culture media?
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Broth + Agar (solidifying agent)
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Blood agar involves...
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Hemolysis and No Hemolysis
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Define: Serial dilution
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to reduce the population side in the inoculum
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What is entailed in the Isolation Technique? 2 things.
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1. Spread plate
2. Streak plate |
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Define the Spread Plate technique:
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Add a drop on the surface of a semisolid media and spread it.
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Define the Streak Plate technique:
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Use inoculating loop, spread sample by making lines on surface of a semi-solid culture media.
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Which is more commonly used? Spread Plate or Streak Plate?
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Streak Plate is commonly used.
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Two aspects to Microscopy:
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1. Magnification - look bigger
2. Resolution - show seperate 2 points (shorter the wavelength, the better the resolution). |
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Most commonly used microscope?
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Bright field microscope.
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Define characteristics of the dark field microscope:
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- dark background that illuminates the specimen.
- exam bacteria (that do not stain) |
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Purpose of the Phase Contrast Microscopy:
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Differentiate objects with different density.
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Characteristics of the Flourescent Microscope:
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Uses fluorescent dye to make the image visible to the human eye.
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Characteristics of electron microscope:
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Uses a beam of Electrons.
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What is the Wet Mount in preparing specimens used for?
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Live samples
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What is the fixes and stained smears used for?
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Permanent Preparation (dry heat and air).
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Types of staining?
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Positive and Negative
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Example of cell that may use negative staining?
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Cocci Cells
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What is the white zone around a vegetative cell? (In negative staining).
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The capsule
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What are the 5 steps in differential staining (in Gram positive)?
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Fixation
Crystal violet iodine treatment decolorization counter stain safranin |
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What is acid fast staining used for?
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Acid fast bacteria.
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Example of acid fast bacteria that would use acid fast staining:
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Mycobacterium sp.
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Cell cultures have a monolayer growth to identify viruses:
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True.
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