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29 Cards in this Set

  • Front
  • Back
Robert Hooke?
Built microscope and examined thin slices of cork.
Limited magnification power.
Leeuwenhoek:
Small lens that could magnify objects.
First to observe living cells, blood, sperm, bacteria, algae, etc.
Year: 1600
Factors affecting seeing (using microscope)
resolution (resolving power)
age of observation. little thought to explain why.
Robert Brown:
every plan cell had a rounded shape. a NUCLEUS. (kernal)
Scleiden:
plant tissues are composed of cells
+
embryonic plants arise from a single cell
Schwann
plants and animals do not resemble one another structurally
Schwann developed unified theory of cell organization. The Cell Theory (1839):
1. consist of 1+ ell
2. basic unit of life.
Virchow: after schwann, found that...
cells arise from division of other, existing cells.
Cell is also the basic unit of reproduction as well as unit of life? T or F?
True.
cell shape and structure gives clues to its
function!
what does biology today entail?
cytology, biochem, genetics
Cytology. define:
cellular structure studies
study of cells
biochem (define):
cell function and structure
genetics (define) importance in cell:
Mendel (austrian monk): genomes, cloning,
Light microscope:
- earliest tool
-
Define Microtome:
developed for rapid and effiecietn preparion of vvery thin tissue slices of bio. samples. dyes, stain. etc.
Limit of resolution:
how far apart adjacent objects must be inorder to distinguish seperate entities.
resolving power in a microscope
ability to see details of structure.
impt. of phase contrast and differential inteference contrast microscopy:
possible to see living cells.
flourenscence microscopy:
detect specific proteins, DNA, etc.
Transition Electron Microscope
and the Scanning Electron Microscope are:
employs a beam of electrons, uses negative staining, shadowing, freeze fracturing, freeze etching,
Enzymes:
Yeast.
chromotpgraphy:
coloumn, mix of molecules are fractioned
electrophoresiis:
electrical field to seperate molecules based on mobilothy
mass spectrometry used for:
size and composition of individual proteins.
Flemming saw chromosomes:
mitosis division process, 'thread like'.
DNA sequencing
genomes (total DNA content of a cell)
scientific method:
- hypothesis to test.
- experiement
- collect data, intrepret
-
scientific method consists of 3 main things:
1. hypothesis
2. theory
3. law