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26 Cards in this Set
- Front
- Back
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What stains or liquids are needed to perform acid fast staining technique? |
Carbol fuchsin, acid-alcohol, and methylene blue |
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What color will non acid-fast microbes appear? |
Blue |
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T/F Ethyl alcohol is used in acid fast staining |
False. Acid alcohol must be used in acid fast staining |
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Are Acid-fast bacteria gram positive or gram negative? |
Gram positive |
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What prevents bacterial invasion of local tissues for normal colonization in the mouth? |
A highly efficient innate host defense system that monitors colonization |
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Are gram positive or gram negative bacteria typically found in the mouth? |
Both gram positive and gram negative bacteria are found in the mouth |
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What liquids are needed to perform gram staining procedure? |
Crystal violet, Gram's iodine, ethanol, and safranin (And distilled water) |
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How long should crystal violet set on smear in Gram staining? |
1 min |
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How long should Gram's iodine set on surface of smear in Gram staining? |
1 min |
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How long should smear be rinsed with ethanol in Gram staining procedure? |
Until ethanol runs clear |
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How long should safranin set on smear in Gram staining? |
45 sec |
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Which media were antiseptic and disinfectant experiments performed on? |
Trypticase soy agar plates |
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What factors do efficiency of antiseptic and disinfectant agents depend on? |
Concentration, length of exposure, type of microbe, and environmental conditions |
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What do bacteriocidal agents do? |
Kill bacteria |
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Why do acid-fast bacteria retain carbol fuchsin stains? |
Large amounts of glycolipids, especially mycolic acids, in the cell wall |
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What do bacteriostatic agents do? |
Stop bacterial growth |
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What are mechanisms of action for disinfectants and antiseptics? |
Oxidation, denaturation of proteins, and inhibition of enzymes |
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Where do synthetic drugs come from? |
They are synthesized in a laboratory and do not originate from living cells |
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Name two pathogenic acid-fast bacteria strains |
M. Tuberculosis and M. Leprae |
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What do alpha type bacteria do on blood agar? |
Partially consume the agar and produce a green-like mass under colony |
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What do beta type bacteria do on blood agar? |
Digest RBCs completely and consume through agar underneath colony |
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What do gamma type bacteria do on blood agar? |
Gamma types are non-hemolytic and do not destruct RBCs. Colonies appear white. |
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Name three hemolytic cells typically carried in the throat |
Streptococcus, bacillococcus, and e. coli |
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What is hemolysis? |
The rupturing and destroying of erythrocytes |
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Which lab media determines if bacteria are hemolytic or not? |
Blood agar |
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T/F Bacillus is non acid-fast |
True |
