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9 Cards in this Set
- Front
- Back
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What happens to NADP in the LIR? |
It acts as an electron acceptor and is reduced. This reaction is catalysed by a dehydrogenase enzyme. |
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How can this be measured? |
DCPIP dye is added to chloroplast extracts/ |
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How does DCPIP work? |
It acts as an electron acceptor like NADP and is reduced. This changes its colour from blue to colourless |
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What does this measure? |
Dehydrogenase activity and as a result, the rate of photosynthesis. |
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How does a colorimeter show DCPIP being reduced? |
As it's reduced absorbance decreases. |
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How do you extract the chloroplasts? |
You grind the leaf in a mortar and pestle and add this to a chilled isolation solution. Then filter this and centrifuge it twice |
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What does the isolation solution contain? |
Sucrose, potassium chloride and phosphate buffer at pH7 |
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How should the colorimeter be set up? |
With a red filter and zeroed on a tube containing the chloroplast solution and distilled water. |
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What can you use this experiment to measure? |
The rate of photosynthesis at different wavelengths of light |
