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16 Cards in this Set
- Front
- Back
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VonKossa
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Stains black for calcium deposits; deposits are usually granular and deeply basophilic
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Alizarin red
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Stains red for calcium deposits; deposits are usually granular and deeply basophilic
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Lugol's iodide (enhanced with sulfuric acid)
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Used for gross staining; when by itself, stains amyoid brown-black; when enhanced with sulfuric acid, stains it blue-black
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Congo red
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Stains amyloid pink to red with apple green birefringence; used for microscopic/histologic staining
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Potassium permanganate
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use to differentiate between primary and secondary amyloidosis; when added after Congo red, secondary amyloid will lose its affinity for Congo red.
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PAS (Periodic Acid Schiff)
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Stains glucose pink; works for amyloid testing because there is some amount of carbohydate in amyloid; unstained forms of glucose form clear vacuoles with irregular boarders.
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Best carmine
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Stains glucose pink
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Sudan red
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Stains lipids red; vacuoles are sharply demarcated
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Oil Red O
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Stains lipids red; vacuoles are sharply demarcated
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Osmitic acid
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Stains lipids black; vacuoles are sharply demarcated
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Anthracosis-carbon particles (exogenous)
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Does not stain but can be seen as a deposit in the lungs (seen in cigarette smokers)
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Lipofuscin (endogenous)
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Stains brown and granular; associated with "wear and tear"-esp. in permanant cells (cardiac and neurons); autoflourescent and acid-fast; antemortem
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Prussian Blue
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Stains hemosiderin blue-black; distinguish from acid hermatin (an artifact) by using neutral buffered formalin: won't get acid hematin; acid hematin is removed by Saturated Alcoholis Picric Acid; hemosiderin is what is seen inside of macrophages in lungs ("heart failure cells"); without staining they are crystalline or granular, golden-yellow to brown, intracytoplasmatic.
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Biliruben (endogenous; heme)
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Sign of antemortem jaundice
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Melanin (endogenous)
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Color is brown to black; antemortem; compare with post-mortem pigment pseudomelanosis, which is greenish-black
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PTAH
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Stains fibrin/cross-striations in muscle black
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