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12 Cards in this Set

  • Front
  • Back
What are the 6 categories of lab diagnostic techniques for cancer?
1. Standard histology
2. Frozen sections
3. Cytologic techniques
4. Immunophenotypic techniques
5. Cytogenetics
6. Molecular techniques
What are the 2 types of immunophenotypic techniques done for cancer diagnosis?
-Flow cytometry
What is the basis of immunohistochemistry?
The application of antibody-based flourescence markers to tissues.
What are 3 things that immunohistochemistry is used for?
-Classifying primary tumors
-Determining metastasis and its site of origin
-Assessing prognosis
What is studied using conventional cytogenetics?
What is conventional cytogenetics used for?
-Proving clonalitiy
-Detecting diagnostic chromosomal abnormalities to classify tumors or determine prognosis
What is the Philadelphia chromosome?
Ch 22
What disease does Philadelphia chromosome cause?
Chronic myelogenous leukemia
What is the principal use of FISH?
Detecting ACQUIRED genetic aberrations
What type of probe is used in FISH?
Nucleic acid probes, labeled with fluorochromes.
What are molecular techniques?
Southern, northern blots
Microarray, gene sequencing
What are molecular techniques used for?
Detecting clonal lymphocyte populations, gene rearrangements, mutations, amplification, etc.