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20 Cards in this Set
- Front
- Back
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VonKossa
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Stain for calcium deposits
stains black |
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Alizarin red
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Stain for calcium deposits
stains red |
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What are the stains used for calcium deposits?
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VonKossa (black)
Alizarin red (red) |
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What are the the stains for Amyloid?
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Lugol’s iodide
enhanced w/ sulfuric acid (gross) Congo red (histo) Potassium permanganate (histo) PAS (Periodic Acid Schiff) (histo) |
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Lugol’s iodide
enhanced w/ sulfuric acid |
Gross staining for presence of amyloid
Lugol’s alone brown-black, w/ sulfuric acid, blue-black |
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Congo red
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histological stain for amyloid
Pink to red w/ apple green birefringence |
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Potassium permanganate
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histological stain for amyloid
Lose affinity for Congo red DIIFERENTIATE b/t primary and secondary by incubating tissue w/ potassium permanganate – secondary (AA protein) will lose affinity for Congo red |
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PAS (Periodic Acid Schiff)
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histological stain for amyloid and glucose
pink Works because there is some amount of carbohydrate in amyloid Unstained forms clear vacuoles with irregular borders (glucose) |
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Best Carmine
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stain for glucose
pink unstained forms clear vacuoles with irregular borders |
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What are the stains for glucose?
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PAS (Periodic Acid Schiff)
Best carmine |
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What are the stains for lipids?
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Sudan Red (red)
Oil red O (red) Osmic Acid (black) |
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Sudan red
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histological stain for lipids
red Vacuoles sharply demarcated |
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Oil red O
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histological stain for lipids
red Vacuoles sharply demarcated |
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Osmic Acid
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Histological stain for lipid
black vacuoles sharply demarcated |
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What is Anthracosis?
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presence of carbon particles in the lung
Is not stained, but can be seen as a deposit in lungs (seen in cigarette smokers) |
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What is Lipofuscin?
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Associated with “wear and tear” – esp. in permanent cells (cardiac & neurons)
brown and granular Autoflourescent and acid-fast Antemortem |
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Prussian blue
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Stain for hemosiderin
Blue-black presence of iron |
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Masson- Fontana
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stain for melanin
black background with white nuclei |
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Phosphotungstic acid hematoxylin (PTAH)
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Stains fibrin
black |
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What does thrichome stain?
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fibrosis
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