P1 Hemostasis Biochem Exam 3

Front 1

where does factor X occur? what do?

Back 1

occurs in clotting in common pathway

activated to Xa,, which converts prothrombin to thrombin

Front 2

what is the importance of vit. K? participate in clotting?

Back 2

cofactor for postrans modification of clotting factors in liver (II, VII, IX, X, protein C and S)
not directly participate

Front 3

what is role of gamma-carboxyglutamate in clotting factor

Back 3

makes each GLU residue divalent so may bind molecule of Ca++,
this makes them hydrophobic and puts them in phoslipid membranes of platelets

Front 4

many activated coagulation factors are enzymes? what class of reactions do they catalyze?

Back 4

protease activity, aka activating zymogens

Front 5

what is anti-hemophilic factor and at what stage does it function?

Back 5

its factor VIII, not enzyme, cofactor of IX, circulates with vWf
function at clotting stage

Front 6

name vitamin K dependant factors

Back 6

II, VII, IX, X
protein C and S

Front 7

how does factor XIII differ from other enzymes in clotting?

Back 7

only non-proteinase enzyme
its a transamidase that crosslinks fibrin

Front 8

name the factors of the common pathway and discus roles

Back 8

factor X activates
prothrombin to thrombin (II to IIa)
which activates fibrinogen to fibrin (I)
Va cofactor accelerates Xa
XIIIa does crosslinkin

Front 9

which 2 proteins of cascade are not enzymes?

Back 9

Va--> for X
, VIII--> for IX
both are cofactors

Front 10

at which amino acid does K carboxylation occur

Back 10

glutamyl acid at N terminus

Front 11

where are the components of cascade synthesized?

Back 11

liver

Front 12

discuss how coagultion is regulated

Back 12

thrombin activates plasminogen
factor XII activate plasminogen
thrombin in presense of thrombomodulin activate protein C
antithrombin III breaks down clots with heparin by binding active site of thrombin
TFPI inhibit VII, X

Front 13

intrinsic/ extrinsic

Back 13

I--> XII, XI, IX
E-->VII, IX
after IX all common

Front 14

therapeutic use
1-streptokinase
2-tissue plasminogen activator TPA
3-urokinase

Back 14

1-cheap clot dissolve, cleave plasminogen to plasmin
2-activate plasmin, found in endothelian cells
3-same as tpa

Front 15

protein C inhibit coagulation how?

Back 15

breaks down factor V and VIII
activated by thrombin

Front 16

what other processes nessesary for hemostasis

Back 16

vessel constriction
fibrinlysis
platelet activation

Front 17

which arachnoid acid metab derived from platelets? which is produced by endothelial cells

Back 17

thromboxane II
prostacylcin

Front 18

what special Alpha 2 macroglobulin?

Back 18

inhibits thrombin, plasmin