Oxidative Phosphorylation

Front 1

ETC=

Back 1

push H+ out and will diffuse across inner mito membrane and atp will be made

Each carrier receives e from a donor and donates to the next carrier in the chain

Front 2

Chain 5 complexes-
complex 1

Back 2

proton pump-gets e- from nadh and will push h+ out and gives e- to Co enzyme Q
net equation=
NADH+Q+5H+@NAD +QH2 + 4H+
e-carriers=NADH to FMN to Fe/S to Q

Front 3

Chain 5 complexes-
Co enzyme Q details

Back 3

lipophilic with isoprene tail and carries e- from complex 1 to complex 3
can cary one or two e-

Front 4

Chain 5 complexes-
Complex 2

Back 4

Not a H+ pump-
FAD picks up electrons here and will drop enzyeme on CoE Q
FAD-Flavin di nucleotide can carry 1 or 2 e-
Net Equation=
FADH+Q@FAD+QH2
e-carriers=FADH to Fe/S cluster to Q
Succinate Deydrongenase is complex 2

Front 5

Chain 5 complexes-
Complex 3

Back 5

Proton pump
Q gives e- from from 1 and 2
Net Equation =
QH2 2 Cyto C ox+H+matrix@Q+2 Cyto Cred +4H+cytoplasm

Front 6

Chain 5 complexes-
Cytochrome C

Back 6

picks up e- from 3 and carries to 5- Cyto C is in inner membrane space

Front 7

Chain 5 complexes-
Complex 4

Back 7

drops 2 e- on osygen and get 2 h20 out
Net equation=
4Cyto Cred +O2+8 H+matrix@4cyto Cox.+ 2h20 +4H+cytoplasm
e-carriers=Cua to heme a tp heme a3 to Cub

Front 8

picture of complex 3

Back 8

reduced QH2 comes into dock site Q0 and drops off bothe e-,q oxidixed in q site gets 1 e- and leaves

Qh2 (another one) drops off e-s , Cyto C leaves with e-,a nd now fully reduced Q leaves

Front 9

Complex 5
ATP synthase
Structure

Back 9

2 parts F0 and F1
F0=a,b,c, units
F1=greek letters alpha, beta, gamma, delta, epsilon
3 pairs of alpha beta held in place by gamma

Front 10

1.)C subunit or C ring
2.)A unit

Back 10

1.)10 units with aspartic acid residue
2=2 half channels

Front 11

Mechanism of rotation

Back 11

1/2 channels fill with h+ and protonate the aspartic acid residue, residue no longer charged, can drop off h+ on other side after 10H+ bound

Front 12

F1 cycle

Back 12

3 alpha beta dimers interface with gamma subunit and can form:
O=open, ATP and ADP pi diffuse in and out
L=ADP + Pi in and nothing else happend
T= ADP Pi are force together to form ATP
T

Front 13

Net yields-
1=Glycolysis
2=TCA
O3=Oxidative Phosphorylation

Back 13

1=2 ATP 2 NADH
2=2 ATP
3=30 ATP, 2.5 ATP per NADH

Front 14

ATP/ADP Translocase

Back 14

Need to get ADP into matrix from cytoplasm to be used by ATPsiynthase- ADP cytosolic binds, conform. change ADP in and ATP out

Front 15

How does glycerol get intot he glycolytic pathway?

Back 15

Glycerol Kinase turns glycerol into glycerol phosphate
Glycerol phosphate dehydrogenase turns Gly pi into dihydroxyacetone (UDAP)