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Intestinal Nematodes

Anclostoma duodenale, Ascaris lumbricoides, Enterobius vermicularis, Necator americanus, Strongyloides stercoralis, Trichinella spiralis, Trichirus trichiura
Tissue and blood nematodes
Brugia malayi, Loa loa, Onchocerca volvulus, Wuchereria bancrofti
Oral cavity of roundworms
Buccal cavity
The outer surface of roundworms; this surface resists digestion
Cuticle
Third stage larva, infective, non- feeding, sheathed. Has a long slender esophagus
Flariform larva
Embryo stage of filarial parasite, usually in the blood or tissue of definitive host. Ingested by arthropod intermediate host
Microfilaria
Roundworms
Nematodes
First stage larva, non-infective, feeding. Has an hourglass shaped esophagus
Rhabitiform larva
Body parts of nematodes
Nerve cord, digestive tract, complex reproductive organs
How to diagnose Ascaris lumbricoides
Eggs in feces
Where do Ascaris lumbricoides develop to become infective?
1 month in soil
How long do Ascaris lumbricoides remain infective?
Years
How do humans acquire Ascaris lumbricoides?
Ingesting infective eggs
Can Ascaris lumbricoides leave the body?
Yes; through nose, mouth or anus
How does Ascaris lumbricoides move about a human body?
Larva from the eggs penetrate the intestinal wall and migrate to the lungs, larva are coughed up and swallowed and return to the intestine to mature
Where would you find Ascaris?
Appalachia
What species is often found with Ascaris?
Trichuris trichiura
Largest adult nematode?
Ascaris. 22-35 cm
Most common intestinal worm infection worldwide, 2nd most common in US
Ascaris
How many eggs do female Ascaris lay a day?
250,000
Fertile eggs
Corticated
Pinworm or Seatworm
Enterobius vermicularis
Diagnostic and infective stage of Enterobius vermicularis
Egg
How does Enterobius vermicularis/ pinworm/ Seatworm lay eggs?
Migrates out the anus to deposit eggs on perianal folds
How long does infectivity of Enterobius vermicularis/ pinworm/ Seatworm last?
A few days
When and how are eggs of Enterobius vermicularis/ pinworm/ Seatworm sought?
Tape prep in the morning
Can Enterobius vermicularis/ pinworm/ Seatworm eggs be found in air?
Yes
Most common worm infection in US
Enterobius vermicularis/ pinworm/ Seatworm
When does Enterobius vermicularis/ pinworm/ Seatworm migrate in body? What does it cause?
At night, bed wetting, sleeplessness, itching
Hookworm
Necator americanus
Where is hookworm found
North and South America, Asia and Africa
North and South America, Asia and Africa
Old world Hookworm
Anclyostoma duodenale
Where is Anclyostoma duodenale found?
North Europe, S America, Asia, Africa and the Caribbean
How can Ancylostoma infect?
Flariform larvae can infect orally and possibly by transmammary or transplacental passage
Diagnostic stage of hookworm
Egg in feces
Development of hookworm
Egg develops to rhabitiform larva in soil (1-2 days); matures to flariform larva (infective)
How does hookworm infection occur?
When flariform larva penetrates the skin; usually on the feet
How does hookworm migrate through the body?
Larva migrates through lungs, coughed up and swallowed and returns to intestines to mature
Hookworms are often found with
Ascaris and Trichuris
How does Strongyloides stercoralis infection occur?
Larva penetrates skin
How does threadworm larva move around the body?
Larva migrates through the blood to the lungs, and from the lungs to the intestine. Larva may also be found in the sputum.
Where does Strongyloides stercoralis reside in the body?
In the intestinal mucosa
Where is Strongyloides found? (Types of regions)
Worldwide in warm areas, tropics and subtropics
How big is Strongyloides?
Only 2-3 mm
Strongyloides Identification
Eggs not usually seen, look similar to Hookworm eggs; recovered with Enterotest capsule Larva must be differentiated from Hookworm Flariform- Strongyloides has short buccal cavity and hourglass shaped esophagus
Trichosis
Trichinella spiralis
What does hookworm consume?
Blood, heavy infections can result in severe blood loss 100 mL/ day
What percentage of human population is infected with hookworm?
25%
What happens if hookworm fecal exam is delayed?
Hatching of eggs with larva
Which worms have a long buccal cavity?
Hookworms
Threadworm
Strongyloides stercoralis
Diagnostic stage of threadworm
Rhabitiform larva
Where do Strongyloides stercoralis eggs hatch?
In the intestinal mucosa
Whose larva can develop to infective stage in the intestine and reinfect the host?
Strongyloides stercoralis
Can live non parasitically in soil
Threadworm
Hookworms are often found with
Ascaris and Trichuris
How does Strongyloides stercoralis infection occur?
Larva penetrates skin
How does threadworm larva move around the body?
Larva migrates through the blood to the lungs, and from the lungs to the intestine. Larva may also be found in the sputum.
Where does Strongyloides stercoralis reside in the body?
In the intestinal mucosa
In what regions is Strongyloides found?
Worldwide in warm areas, tropics and subtropics
How big is Strongyloides?
Only 2-3 mm
Strongyloides Identification
Eggs not usually seen, look similar to Hookworm eggs; recovered with Enterotest capsule Larva must be differentiated from Hookworm Flariform- Strongyloides has short buccal cavity and hourglass shaped esophagus
Trichosis
Trichinella spiralis
What does hookworm consume?
Blood, heavy infections can result in severe blood loss 100 mL/ day
What percentage of human population is infected with hookworm?
25%
What happens if hookworm fecal exam is delayed?
Hatching of eggs with larva
Which worms have a long buccal cavity?
Hookworms
Threadworm
Strongyloides stercoralis
Diagnostic stage of threadworm
Rhabitiform larva
Where do Strongyloides stercoralis eggs hatch?
In the intestinal mucosa
Whose larva can develop to infective stage in the intestine and reinfect the host?
Strongyloides stercoralis
Can live non parasitically in soil
Threadworm
Hookworms are often found with
Ascaris and Trichuris
How does Strongyloides stercoralis infection occur?
Larva penetrates skin
How does threadworm larva move around the body?
Larva migrates through the blood to the lungs, and from the lungs to the intestine. Larva may also be found in the sputum.
Where does Strongyloides stercoralis live?
In the intestinal mucosa
Where is Strongyloides found?
Worldwide in warm areas, tropics and subtropics
How big is Strongyloides?
Only 2-3 mm
Strongyloides Identification
Eggs not usually seen, look similar to Hookworm eggs; recovered with Enterotest capsule Larva must be differentiated from Hookworm Flariform- Strongyloides has short buccal cavity and hourglass shaped esophagus
Trichosis
Trichinella spiralis
Zoonotic disease, carnivorous mammals are the primary host
Trichosis
Diagnostic stage is larva encysted in striated muscle "nurse cells"
Trichinella spiralis
What does hookworm consume?
Blood, heavy infections can result in severe blood loss 100 mL/ day
What percentage of human population is infected with hookworm?
25%
What happens if hookworm fecal exam is delayed?
Hatching of eggs with larva
Which worms have a long buccal cavity?
Hookworms
Threadworm
Strongyloides stercoralis
Diagnostic stage of threadworm
Rhabitiform larva
Where do Strongyloides stercoralis eggs hatch?
In the intestinal mucosa
Whose larva can develop to infective stage in the intestine and reinfect the host?
Strongyloides stercoralis

Can live non parasitically in soil

Threadworm

Ascaris lumbricoides egg

Ascaris lumbricoides egg

Ascaris lumbricoides egg

Enterobius vermicularis

Enterobius vermicularis

Enterobius vermicularis

Hookworm

Hookworm

Hookworm

Ascaris infection

Hookworm egg

Hookworm egg

Hookworm larva

Hookworm larva

Strongyloides stercoralis

Strongyloides stercoralis

Strongyloides stercoralis

Trichuris trichuria egg

Trichuris trichuria egg

Trichuris trichuria egg

Wucheria bancroft

Wucheria bancroft

Brugia malayi

Brugia malayi

How does Strongyloides infect a human?
Larva penetrates skin
How does Strongyloides move about the body?
Larva migrates the blood to the lungs, and from the lungs to the intestine , larva may also be found in sputum
How big are Strongyloides stercoralis?
2-3mm
US infection rate of Trichinella spiralis
~4%
Distribution of Trichinella spiralis
World-wide in meat eating populations, but rare in the tropics
Trichuris trichiura
Whipworm
How to diagnose Trichuris trichiura
Eggs in feces
Where do Trichuris trichiura eggs mature?
In soil, infective in one month
How do humans acquire Trichuris trichiura infection?
Eating eggs
How does whipworm travel through body?
Larva hatch in small intestine, develop in villi and mature in colon
Who has Barrel shaped eggs with plugged ends and a smooth surface
Trichuris trichiura
Which worm produces living embryos, microfilariae, that migrate into lymphatics , blood or skin
Filariae
Filariae intermediate host is who and does what
Arthropod ingests microfilariae
Where do adult Strongyloides live?
In the intestinal mucosa
Who shows periodicity and why
Microfilariae become more prevalent at certain times during the day- these seem to correlate with the feeding patterns of the arthropod hosts
Where is Strongyloides found?
In warm areas, tropics and subtropics
Strongyloides Identification
Eggs: not usually seen, look similar to hookworm eggs, may be recovered using Entero-Test capsule. Larvae: must differentiate between Strongyloides rhabitiform larva and Hookworm Filariform larva.
Characteristics of Strongyloides larva
Short buccal cavity, hourglass shaped esophagus
Zoonotic disease, carnivorous mammals are the primary hosts
Trichosis
Diagnostic stage of Trichinella spiralis
Larva encysted in striated muscle "nurse cells"
How does Trichinella spiralis infection occur?
Undercooked infected meat is eaten
How long must infected meat be frozen to kill Trichinella spiralis?
20 days
Trichinella spiralis movement through the body
Larva is freed when meal is digested, rapidly develop into adults. Adults live on intestine. Female releases larva into submucosa. Larva disseminate via the blood stream
Knott's technique
Blood can be concentrated and the RBCs lysed before staining to concentrate microfilariae
How is microfilariae identified
By the presence or absence of a sheath around the larva and whether there are nuclei present in the tip of the tail and if there is a pattern to their distribution
Diagnostic stage of Filariae
Microfilariae
Adults live in subcutaneous tissue, migrate actively
Loa loa; eyeworm
Loiasis/ Calabar swellings
Loa loa
Elephantiasis a
Wucheria bancrofti
Adults live in the lymphatics, blockage causes extreme edema
Wuchereria bancrofti
Wucheria bancrofti intermediate host
Mosquito
Microfilariae have a sheath but have no nuclei in tail tupmicto
Wucheria bancrofti
Microfilariae are found in the blood
Wucheria bancrofti
Tightly coiled, sheath present, nuclei tightly packed, terminal and sub- terminal nuclei extend to tip of the tail. Adults live in lymphatics. Mosquito arthropod host.
Brugia malayi
Eyeworm
Loa loa
Loa loa arthropod host
Chrysops (Deerfly)
Sheath present nuclei present in tail, continuous
Loa loa
Diagnostic stage of Filariae
Microfilariae
Adults live in subcutaneous tissue, migrate actively
Loa loa; eyeworm
Loiasis/ Calabar swellings
Loa loa
Elephantiasis a
Wucheria bancrofti
Adults live in the lymphatics, blockage causes extreme edema
Wuchereria bancrofti
Wucheria bancrofti intermediate host
Mosquito
Microfilariae have a sheath but have no nuclei in tail tupmicto
Wucheria bancrofti
Microfilariae are found in the blood
Wucheria bancrofti
Tightly coiled, sheath present, nuclei tightly packed, terminal and sub- terminal nuclei extend to tip of the tail. Adults live in lymphatics. Mosquito arthropod host.
Brugia malayi
Eyeworm
Loa loa
Loa loa arthropod host
Chrysops (Deerfly)
Sheath present nuclei present in tail, continuous
Loa loa
Black fly is the arthropod host. Major cause of blindness in Africa
Onchocerca volvulus
Adults live in subcutaneous tissue, nodules form around the adult
Onchocerca volvulus
River blindness
Onchocerciasis
Black fly is the arthropod host. Major cause of blindness in Africa
Onchocerca volvulus
Adults live in subcutaneous tissue, nodules form around the adult
Onchocerca volvulus
River blindness
Onchocerciasis