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11 Cards in this Set

  • Front
  • Back
How do we observe the gross morphology of fungus cultures?
1. Rate of growth
2. General typography
3. Texture
4. Surface pigmentation
5. Pigmentation on reverse
Describe different types of 'typography' of mycological cultures?
- flat
- heaped
- regularly folded
- irregularly folded
Describe example descriptors of texture
- yeast like
- glabrous
- powdery
- granular
- velvety
-cottony
Describe the wet mount method for fungi
1. sterile technique
2. excavate a edge colony trying to minimise the amount of agar accompanying the undisturbed colony
3. transfer to slide washing off colony with 70% ethanol
4. tease out mycelium with the wire nd the corner of a coverslip
...
1. sterile technique
2. excavate a edge colony trying to minimise the amount of agar accompanying the undisturbed colony
3. transfer to slide washing off colony with 70% ethanol
4. tease out mycelium with the wire nd the corner of a coverslip
5. Add drop of Lactophenol blue and cover with coverslip when ethanol has mostly evaporated
6. blot excess dye
7. microscopy
What other name does the wet mount method do by?
LPCB
Why is 70% ethanol used in a WET MOUNT?
Move loose spores to corner of drop
To remove culture from nichrome wire
Describe PDA agar
potato dextrose agar
Identify
Identify
Mucor spp.
- aseptate
- height of several cm resembles cotton candy.  white initially and grayish brown in time. From the reverse, it is white. 
-
Mucor spp.
- aseptate
- height of several cm resembles cotton candy. white initially and grayish brown in time. From the reverse, it is white.
-
Describe the sticky tape method
- put a small drop of lactophenol blue
- place sticky tape between thumb and forefinger, push down on agar with the back of a pencil back and forth several times and lift,
- place on top of drop of dye
- examine
What are the downsides to the sticky tape method
loose spores
Identify
Identify
Microsporum gypseum
Microsporum gypseum