Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
11 Cards in this Set
- Front
- Back
How do we observe the gross morphology of fungus cultures?
|
1. Rate of growth
2. General typography 3. Texture 4. Surface pigmentation 5. Pigmentation on reverse |
|
Describe different types of 'typography' of mycological cultures?
|
- flat
- heaped - regularly folded - irregularly folded |
|
Describe example descriptors of texture
|
- yeast like
- glabrous - powdery - granular - velvety -cottony |
|
Describe the wet mount method for fungi
|
1. sterile technique
2. excavate a edge colony trying to minimise the amount of agar accompanying the undisturbed colony 3. transfer to slide washing off colony with 70% ethanol 4. tease out mycelium with the wire nd the corner of a coverslip 5. Add drop of Lactophenol blue and cover with coverslip when ethanol has mostly evaporated 6. blot excess dye 7. microscopy |
|
What other name does the wet mount method do by?
|
LPCB
|
|
Why is 70% ethanol used in a WET MOUNT?
|
Move loose spores to corner of drop
To remove culture from nichrome wire |
|
Describe PDA agar
|
potato dextrose agar
|
|
Identify
|
Mucor spp.
- aseptate - height of several cm resembles cotton candy. white initially and grayish brown in time. From the reverse, it is white. - |
|
Describe the sticky tape method
|
- put a small drop of lactophenol blue
- place sticky tape between thumb and forefinger, push down on agar with the back of a pencil back and forth several times and lift, - place on top of drop of dye - examine |
|
What are the downsides to the sticky tape method
|
loose spores
|
|
Identify
|
Microsporum gypseum
|