Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
15 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
Learning objective 2 |
Describe the types of vectors used in gene cloning |
|
|
|
Accepts inserts of up to 15 kb |
plasmids |
|
|
|
YAC |
Yeast artificial chromosomes |
|
|
|
Viruses that infect bacteria |
Bacteriophages |
|
|
|
BAC |
Bacterial artificial chromosomes |
|
|
|
Example: lamda virus of E.coli |
Bacteriophages |
|
|
|
Accepts inserts of 100-2000 kb |
YAC |
|
|
|
Genetically modified yeast chromosomes which can accept very large DNA fragments |
YAC
Full name? |
Yeast artificial chromosomes |
|
|
Very large DNA plasmids modified to carry a DNA fragment |
BAC
Full name |
bacterial artificial chromosomes |
|
|
Circular double-stranded DNA molecules that replicate outside of the main chromosome in bacteria or yeast |
plasmids |
|
|
|
Accepts inserts up to 45 kb |
cosmids |
|
|
|
Accepts inserts of 100-300 kb |
BAC |
|
|
|
Modified plasmids that can hold larger pieces of DNA |
Cosmids |
|
|
|
Accepts inserts of upt o 20 kb |
Bacteriophages |
|
|
|
Accepts inserts
- up to 15 kb - up to 20 kb - up to 45 kb - 100-300 kb - 100-2000 kb |
- up to 15 kb: Plasmid - up to 20 kb: Bacteriophages, example? - up to 45 kb: Cosmids - 100-300 kb: BAC, full name? - 100-2000 kb: YAC, full name? |
Bacteriophages: Lambda virus of E.coli
Bacterial artificial chromosome: BAC
Yeast artificial chromosome: YAC |
