Msu Bmb 461 Glycolysis

Front 1

The net production of ATP and NADH, respectively, per glucose during the 10 reactions of glycolysis is:

Back 1

2 ATP and 2 NADH

Front 2

One direct product of the phosphofructokinase 1 (PFK-1) during glycolysis is:

Back 2

fructose 1,6-bisphosphate

Front 3

Three of the following enzymes catalyze three consecutive reactions during glycolysis, list in order: 1. enolase 2. hexokinase 3. phosphofructokinase 1 4. phosphoglycerate mutase 5. glyceraldehyde 3-phosphate dehydrogenase 6. phosphohexose isomerase

Back 3

hexokinase, phosphohexose isomerase, phosphofructokinase 1

Front 4

In the direction of glycolysis the products of pyruvate kinase reaction are:

Back 4

pyruvate, ATP

Front 5

The enzyme catalyzing the conversion of 3-phosphoglycerate to 2-phosphoglycerate in glycolysis is:

Back 5

phosphoglycerate mutase

Front 6

Ignoring the preparatory phase, during the pay-off phase of glycolysis the following will be produced:

Back 6

2 pyruvate, 4 ATP, 2 NADH, 2H+, 2H2O

Front 7

If 4-[14C]-glucose (carbon 4 of glucose is labeld) is fed to yeast and glycolytic intermediates are analyzed shortly thereafter, which carbon is found labeled in 50% of the glyceraldehyde 3-phosphate molecules?

Back 7

C1

Front 8

Which is not true?
1. Tumors show increased glycolytic activity
2. Reduced glycolytic activity is observed in hepatocytes during fasting conditions.
3. The brain depends on glycolysis for energy production
4. Reduced glycolytic activity is a cause of tumor formation.
5. Yeast depends on glycolysis under anaerobic conditions.
6. Myocytes depend on glycolysis for energy production under anaerobic conditions.
7. Glycolysis is an ancient process predating the presence of oxygen in the atmosphere.

Back 8

Reduced glycolytic activity is NOT a cause for tumor formation.

Front 9

Which of the following is not correct about glycolysis under anaerobic conditions.
1. It is the basis for the fermentation of sugars to ethanol in yeast.
2. It leads to the production of lactate in muscles.
3. It is responsible for the carbonation of beer, because decarboxylation of pyruvate produces CO2.
4. It is indirectly responsible for muscle cramps after prolonged activity.
5. It leads to decreased glucose consumption in yeast, also called the Pasteur effect.
6. It requires reactions other than respiration for the regeneration of NAD+

Back 9

It DOES NOT lead to decreased glucose consumption in yeast, also called the Pasteur effect

Front 10

Which enzyme converts UDP-galacose to UDP-glucose

Back 10

UDP-glucose epimerase

Front 11

Gluconeogenesis bypasses three exerconic reaction of glucose, they are:

Back 11

phosphofructokinase 1, pyruvate kinase, hexokinase

Front 12

In hepatocytes gluconeogenesis starting from pyruvate requires mitochondrial transporters for

Back 12

pyruvate and malate

Front 13

Gluconeogenesis is costly because it requires the following nucleotides to produce one mole of glucose from 2 moles of pyruvate:

Back 13

2 GTP, 4 ATP, 2 NADH

Front 14

Persons with specific mutations in the gene for glucose 6-phosphate dehydrogenase give rise to a less active version of the the enzyme:

Back 14

are naturally resistant to the malaria parasite

Front 15

During the oxidative phase of the pentose phosphate pathway which carbon of 6-phhosphogluconate is released as carbon dioxide during the 6-phosphogluconate dehydrogenase reaction?

Back 15

C1

Front 16

What feature is observed for hexokinase IV in liver cells but not other hexokinase isoforms in muscle cells.

Back 16

It has a relatively high Km for glucose.

Front 17

A key regulated enzyme of glycolysis is phosphofructokinase 1, it is activated by:

Back 17

AMP, ADP, and fructose 2,6 bisphosphate

Front 18

The branching of glycogen serves to:

Back 18

reduce the number of reducing ends

Front 19

Which protein is permanently and covalenlty linked to the glycogen particle.

Back 19

glycogenin