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5 Cards in this Set
- Front
- Back
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Aims of immunomolecular analyses |
-Diagnostic application (define origin of metastases from unknown primary tumours, to better classify it) -Prognostic application (evaluation of proliferative index of a neoplasm, e.g. genetic lesion) -Therapeutic application (molecular defect identification, e.g. monoclonal Ab, Kinase inhibitors) |
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What are Cytokeratins (CK)? |
Intermediate filaments expressed in all epithelial cells and epithelial-derived neoplasm. 19 different types specific in different epithelial. Identification can help in diagnosis of epithelial neoplasm |
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Differential CK expression in diagnosis of solid tumours |
-> CK7+/CK20+ Bladder epithelium -> CK7+/CK20- Lung, breast, ovary -> CK7-/CK20+ Colon, stomach -> CK7-/CK20-/CK8+ Liver, prostate, kidney |
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Applications of Immunohistochemistry |
- Comfirmation of morphological diagnosis - Differential diagnosis - Search from prognostic markers - Identification of minimal residual disease (after therapy) - Search for potential therapeutic agent |
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Methods of molecular biology analysis |
- In situ hybridization - PCR - Sequence analysis (Sanger, NGS, ...) - Gene expression with microarrays |
