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15 Cards in this Set
- Front
- Back
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Antibody |
Protein produced by the immune system toidentify and neutralize foreign objects |
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Antigen |
Any substance capable of eliciting an adaptive immune response (production of a Ab) |
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Monoclonal Antibody |
Antibodies secreted from a SINGLE B-Cell, has identical PARATOPES |
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Paratope |
Region of the antibody that binds the epitope (hypervariable region/Complementarity determining region/CDR) |
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Epitope |
Antigen Binding site |
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Hybridoma (creation and use) |
1.) Spleen cells from mouse immunized w/ antigen = B cells formed 2.) B cells and Myeloma cells combined in PEG w/ HAT culture medium 3.) Hybridomas removed, purified and ensured the produce correct Ab 4.) Hybridoma cultured to grow MAb |
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-Mo nab suffix |
100% Murine Derived MAb Results in HAMA response Human Anti-Murine-Antibody |
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-Xi mab suffix |
Chimeric 65% human Variable regions= Murine Fc= Human |
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-Zu mab Suffix |
Humanized 90% Human Hypervariable Regions = Murine Fc+ parts of variable region = human |
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- U mab suffix |
Fully Human 100% (normally made in transgenic mice though) |
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Administration of MAb's |
IV, SC, or IM (never oral) |
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Therapeutic Uses of MAbs (4) |
1.) Direct Modulation of target antigen (apoptosis or inhibition of cell signaling) 2.) CDC 3.) ADCC 4.) Delivery of a cytotoxic agent |
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Fc Interaction w/ an FcR (I, II or III) = signals for ____? |
ADCC |
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Fc Interaction w/ C1q, C1r or C1s = Signals for ____? |
CDC |
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Common MAB ADRs? |
Allergic Rxn, Flu-like symtoms, N/V/D, HA, Hypo/hyperTN |
