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4 Cards in this Set
- Front
- Back
Methods to Assess Tumors for Mutations, etc. |
Chromosomes: Karyotype, Array CGH, FISH Sub-microscopic DNA mutations: Allele specific PCR, qRT PCR, Pyrosequencing, Southern Blot/MLPA, Sanger sequencing, Next gen. seq. Epigenetic: Hypermethylation Analysis RNA: Expression arrays, Rev. Transcriptase PCR Protein: Immunohistochemistry |
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Detection of molecular biomarkers in order to |
- Detect cancer - Determine prognosis - Monitor disease progression - Monitor therapeutic response |
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NGS (Next Generation Sequencing) / Massively Parallel Sequencing |
Genomic DNA -> fragmented library of DNA pieces all sequenced at the same time -> individual reads aligned to reference sequence => whole-genome sequence (~ consensus of aligned reads) |
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Personalized Medicine |
Is the best. |