Molecular Biology Chapter 22 Fill In The Blank

Front 1

Cloning vectors have selectable markers like the ______ that makes Beta-lactamase

Back 1

bla or amp gene

Front 2

_______ is created by reverse transcriptase

Back 2

complementary DNA (cDNA)

Front 3

Certain cloning vectors are also called _____ when the cloned insert is converted into mRNA and protein.

Back 3

expression vectors

Front 4

In _______, Beta-galactosidase is expressed as two protein subunits that come together in the cytoplasm to cleave X-gal and produce a blue colored bacterial colony.

Back 4

alpha complementation

Front 5

Restriction enzymes are a type of ______ or enzyme that cuts DNA in the middle stand.

Back 5

endonuclease

Front 6

Restriction enzyme sites are usually _____ where the first nucleotides on the sense strand are the same as the second half nucleotides on the antisense strand

Back 6

endonuclease

Front 7

_____ are identified when one organism has a particular restriction enzyme site that is missing in a closely related organism.

Back 7

Restriction fragment length polymorphisms (RFLPs)

Front 8

Eukaryotic mRNA has a poly(A) tail which is used to isolate the mRNA from the remaining cellular components by binding to ____ or _____ column or beads.

Back 8

oligo (dT); olgio(U)

Front 9

Inserts or DNA fragments or interest are added to the _____ or of a cloning vector because this region has many unique restriction enzyme sites near each other.

Back 9

polylinker; multiple cloning site

Front 10

______vectors have lambda cos sites flanking a large piece of genomic DNA.

Back 10

Cosmid