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4 Cards in this Set
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- Back
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Things you need in Sanger DNA sequencing
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DNA Template
Primer the 4 deoxyribonucleotides 4 dideoxyribonucleotide (one of each dCTP, dATP, cGTP, cTTP in separate tubes) |
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DNA sequencing limits
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can't be more than 1200 bases
DNA has to be clean |
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Sanger's method gives the original or complementary strand of the template?
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It gives the compliment!
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How is RNA different than DNA?
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RNA is single stranded, has uracil instead of thymine, has ribose instead of deoxyribose.
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